Kikuchi H, Sagami I, Fujii H, Ohmachi T, Watanabe M
Department of Cancer Chemotherapy and Prevention, Tohoku University, Sendai, Japan.
Tohoku J Exp Med. 1990 Apr;160(4):323-32. doi: 10.1620/tjem.160.323.
Cytochrome P-450MC was induced in pulmonary microsomes of 3-methylcholanthrene-treated rats and also at low level in that of isosafrole-treated rats. Cytochrome P-450d was not detected in the lungs of 3-methylcholanthrene- or isosafrole-treated rats by the method of Western blot analysis with a polyclonal antibody raised against cytochrome P-450c which is equally effective against P-450d, nor by the method of Northern hybridization probed with pcP450mc3 (P-450d probe). Complementary DNA of P-450MC was isolated from rat pulmonary cDNA library and the nucleotide sequence of pulmonary cDNA was compared with that of hepatic P-450c cDNA reported by Yabusaki et al. There was no gross change in nucleotide sequences of cDNA between pulmonary P-450MC and hepatic P-450c.
细胞色素P - 450MC在经3 - 甲基胆蒽处理的大鼠肺微粒体中被诱导，在经异黄樟素处理的大鼠肺微粒体中也有低水平诱导。通过使用针对细胞色素P - 450c产生的多克隆抗体进行蛋白质印迹分析方法（该抗体对P - 450d同样有效），以及用pcP450mc3（P - 450d探针）进行Northern杂交的方法，在经3 - 甲基胆蒽或异黄樟素处理的大鼠肺中均未检测到细胞色素P - 450d。从大鼠肺cDNA文库中分离出P - 450MC的互补DNA，并将肺cDNA的核苷酸序列与Yabusaki等人报道的肝P - 450c cDNA的核苷酸序列进行比较。肺P - 450MC和肝P - 450c的cDNA核苷酸序列没有明显变化。
