Kano Y, Nakura K, Nakane T, Ueda Y, Uemura Y, Yokoyama K, Ishida H, Ohyanagi H, Saitoh Y
Central Research Laboratories, Green Cross Corporation, Hirakata, Japan.
Tohoku J Exp Med. 1990 Apr;160(4):361-73. doi: 10.1620/tjem.160.361.
We have evaluated approximately 10,000 monoclonal antibodies resulting from 14 hybridization of spleen cells from mice immunized with established cancer cell lines. They were screened by binding assays using cancer cell lines or normal fibroblast cells, followed by immunohistochemical study on tissue sections. Ninety-one monoclonal antibodies were obtained by above binding assays. One of them, named KM10 was further investigated. Isotype of KM10 was IgG1 with kappa-light chain. It was shown that KM10 has strong reactivity with tumors of colon, stomach, papilla Vater, and pancreas, while the limited reactivity displayed with normal tissues. The electron microscopic observation revealed that the antigen recognized by KM10 is expressed on the surface of cancer cells. KM10 could mediate ADCC. These results indicate that KM10 is a good candidate for a probe of diagnosis and therapy of cancer.
我们评估了约10,000种单克隆抗体，这些抗体来自用已建立的癌细胞系免疫的小鼠脾细胞进行的14次杂交。通过使用癌细胞系或正常成纤维细胞进行结合试验对它们进行筛选，随后对组织切片进行免疫组织化学研究。通过上述结合试验获得了91种单克隆抗体。其中一种名为KM10的抗体被进一步研究。KM10的同种型为带有κ轻链的IgG1。结果表明，KM10与结肠、胃、 Vater乳头和胰腺的肿瘤具有强烈反应性，而与正常组织的反应性有限。电子显微镜观察显示，KM10识别的抗原在癌细胞表面表达。KM10可介导ADCC。这些结果表明，KM10是癌症诊断和治疗探针的良好候选者。
