Li Ping, Zhao Guo-Qiang, Chen Teng-Fei, Chang Jing-Xia, Wang Hua-Qi, Chen Shan-Shan, Zhang Guo-Jun
Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan , People's Republic of China and.
J Asthma. 2013 Nov;50(9):960-4. doi: 10.3109/02770903.2013.822080. Epub 2013 Aug 16.
To investigate the expression of serum miRNA-21(miR-21) and miRNA-155 (miR-155) in idiopathic pulmonary fibrosis (IPF).
A study including 65 patients with IPF and 65 similar age and gender healthy controls was performed. Serum specimens were collected from all subjects. Total RNA was extracted and the quantitative reverse transcription-polymerase chain reaction was used to measure serum miR-21 and miR-155 in both groups. Clinicopathologic features were assessed to determine associations with serum miR-21 and miR-155 concentrations.
Serum miR-21 expression was significantly higher in IPF samples than in healthy controls (p < 0.01), while serum miR-155 expression did not show a statistically significant difference (p > 0.05). Forced vital capacity (FVC) and radiologic features were associated with miR-21 and miR-155 expression in serum (p < 0.05). Neither miR-21 nor miR-155 expression was statistically significantly associated with clinicopathologic parameters, such as gender (p > 0.05) and age (p > 0.05).
These findings suggest that serum miR-21 is associated with IPF and the degree of damage indicated by FVC and radiologic examinations could correlate with miR-21 and miR-155 expression in serum. From another perspective, our study confirmed serum miRNA can be stable and detectable in serum of patients with IPF, which could prove useful as it could be considered as a new biomarker in serum for diagnosis and assessment of prognosis of IPF in the future.
研究血清微小RNA-21（miR-21）和微小RNA-155（miR-155）在特发性肺纤维化（IPF）中的表达情况。
开展一项研究，纳入65例IPF患者以及65例年龄和性别相仿的健康对照者。采集所有受试者的血清标本。提取总RNA，并采用定量逆转录-聚合酶链反应检测两组血清中的miR-21和miR-155。评估临床病理特征以确定其与血清miR-21和miR-155浓度的相关性。
IPF样本中血清miR-21的表达显著高于健康对照者（p < 0.01），而血清miR-155的表达未显示出统计学显著差异（p > 0.05）。用力肺活量（FVC）和影像学特征与血清中miR-21和miR-155的表达相关（p < 0.05）。miR-21和miR-155的表达与性别（p > 0.05）和年龄（p > 0.05）等临床病理参数均无统计学显著相关性。
这些研究结果表明，血清miR-21与IPF相关，且FVC和影像学检查所提示的损伤程度可能与血清中miR-21和miR-155的表达相关。从另一个角度来看，我们的研究证实血清微小RNA在IPF患者血清中可以稳定存在并可检测到，这可能具有重要意义，因为它未来有可能被视为血清中用于IPF诊断和预后评估的新型生物标志物。
