成熟绵羊采食不同水平的紫花苜蓿草块时内脏组织的能量代谢。
Energy metabolism by splanchnic tissues of mature sheep fed varying levels of lucerne hay cubes.
机构信息
Graduate School of Biosphere Science, Hiroshima University, Higashihiroshima-shi, 739-8528, Japan.
出版信息
Animal. 2013 Oct;7(10):1622-30. doi: 10.1017/S1751731113001225. Epub 2013 Jul 3.
The objective of this study was to determine the pattern of energy metabolites net flux across the portal-drained viscera (PDV) and total splanchnic tissues (TSP) in mature sheep fed varying levels of lucerne hay cubes. Four Suffolk mature sheep (61.4 ± 3.6 kg BW) surgically fitted with multi-catheters were fed four levels of dry matter intake (DMI) of lucerne hay cubes ranging from 0.4- to 1.6-fold the metabolizable energy (ME) requirements for maintenance. Six sets of blood samples were simultaneously collected from arterial and venous catheters at 30-min intervals. With increasing DMI, apparent total tract digestibility increased linearly and quadratically for dry matter (P < 0.05), quadratically (P < 0.05) with a linear tendency (P < 0.1) for organic matter and tended to increase quadratically (P < 0.1) for NDF. PDV release of volatile fatty acids (VFA) and β-hydroxybutyric acid was relatively low at 0.4 M and then linearly increased (P < 0.05) with increasing DMI. Net PDV flux of non-esterified fatty acids showed curvilinear decrease from 0.4 to 1.2 M and then increased at 1.6 M. The respective proportions of each VFA appearing in the portal blood differed (P < 0.05) with DMI and this difference was more obvious from 0.4 to 0.8 M than from 0.8 to 1.6 M. Heat production, as a percentage of ME intake (MEI), decreased linearly (P < 0.05) with increasing DMI accounting for 37%, 21%, 16% and 13% for PDV and 62%, 49%, 33% and 27% for TSP at 0.4, 0.8, 1.2 and 1.6 M, respectively. As a proportion of MEI, total energy recovery including heat production, decreased linearly with increasing DMI (P < 0.05) accounting for 113%, 83%, 62% and 57% for PDV and 140%, 129%, 86% and 83% for TSP at 0.4, 0.8, 1.2 and 1.6 M, respectively. Regression analysis revealed a linear response between MEI (MJ/day per kg BW) and total energy release (MJ/day per kg BW) across the PDV and TSP, respectively. However, respective contributions of energy metabolites to net energy release across the PDV and TSP were highly variable among treatments and did not follow the same pattern of changes in DMI.
本研究旨在确定绵羊在不同水平的紫花苜蓿干草立方体喂养下，穿过门静脉引流内脏（PDV）和总内脏组织（TSP）的能量代谢物净流量模式。4 只苏塞克斯成熟绵羊（61.4 ± 3.6 kg BW）接受了多导管手术，接受了 4 种不同水平的干物质摄入量（DMI），紫花苜蓿干草立方体的干物质摄入量从维持代谢能（ME）需求的 0.4 倍到 1.6 倍不等。以 30 分钟为间隔，从动脉和静脉导管同时采集 6 组血液样本。随着 DMI 的增加，干物质（P < 0.05）和有机物（P < 0.05）的表观全肠道消化率呈线性和二次增加，NDF 呈二次增加（P < 0.1），有线性趋势（P < 0.1）。挥发性脂肪酸（VFA）和β-羟丁酸在 0.4 M 时相对较低，然后随着 DMI 的增加呈线性增加（P < 0.05）。非酯化脂肪酸的净 PDV 流量从 0.4 到 1.2 M 呈曲线下降，然后在 1.6 M 时增加。出现在门静脉血液中的每种 VFA 的比例不同（P < 0.05），并且从 0.4 到 0.8 M 的差异比从 0.8 到 1.6 M 的差异更明显。以 ME 摄入量（MEI）的百分比表示的产热量线性下降（P < 0.05），0.4、0.8、1.2 和 1.6 M 时 PDV 分别占 37%、21%、16%和 13%，TSP 分别占 62%、49%、33%和 27%。作为 MEI 的一部分，包括产热在内的总能量回收呈线性下降（P < 0.05），0.4、0.8、1.2 和 1.6 M 时 PDV 分别占 113%、83%、62%和 57%，TSP 分别占 140%、129%、86%和 83%。回归分析显示，MEI（MJ/天/公斤 BW）与 PDV 和 TSP 之间的总能量释放（MJ/天/公斤 BW）之间存在线性关系。然而，能量代谢物对 PDV 和 TSP 净能量释放的各自贡献在处理之间高度可变，并且与 DMI 的变化模式不同。
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