Warth J A, Desforges J F, Stolberg S
Br J Haematol. 1977 Nov;37(3):373-7. doi: 10.1111/j.1365-2141.1977.tb01008.x.
We have documented a rise in pCO2 when erythrocytes are haemolysed by freeze-thaw using solid CO2 and methanol. This is partially corrected by preventing leakage of CO2 into the system. The remaining increment is partially explained by stimulation of the hexose monophosphate shunt when erythrocytes are haemolysed by this technique.
我们已经记录到,当使用固态二氧化碳和甲醇通过冻融使红细胞溶血时,二氧化碳分压(pCO2)会升高。通过防止二氧化碳泄漏到系统中,这种情况会得到部分纠正。当用这种技术使红细胞溶血时,己糖磷酸旁路的刺激可部分解释剩余的增量。