Monzón C M, Mancebo O A, Roux J P
National Council for Scientific and Technical Research (CONICET), Argentina CEDIVEF, Formosa.
Vet Parasitol. 1990 May;36(1-2):141-6. doi: 10.1016/0304-4017(90)90102-h.
In a total of 165 blood samples from horses in the Province of Formosa (Argentina), the diagnosis for equine trypanosomiasis (T. evansi) was made using Giemsa-stained smears (GSS), wet blood films (WBF), Strout's concentration method (SCM), haematocrit centrifuge technique (HCT), buffy coat method (BCM) and mouse inoculation of blood (MBI). Trypanosoma evansi was demonstrated in 52 samples. Mouse inoculation gave a sensitivity of 88.2%; HCT 71.1%; BCM 63.4%; WBF 53.8%; SCM 46.1% and GSS 45.6%. No single method alone was totally effective. The haematocrit centrifuge technique, mouse inoculation of blood and Giemsa-stained smears were proposed as the most effective diagnostic combination.
在从福尔摩沙省（阿根廷）的马匹采集的总共165份血样中，使用吉姆萨染色涂片（GSS）、湿血膜（WBF）、斯特劳特浓缩法（SCM）、血细胞比容离心技术（HCT）、血沉棕黄层法（BCM）和小鼠血液接种法（MBI）对马锥虫病（伊氏锥虫）进行诊断。在52份样本中检测到伊氏锥虫。小鼠血液接种法的灵敏度为88.2%；血细胞比容离心技术为71.1%；血沉棕黄层法为63.4%；湿血膜为53.8%；斯特劳特浓缩法为46.1%；吉姆萨染色涂片为45.6%。没有一种单独的方法是完全有效的。血细胞比容离心技术、小鼠血液接种法和吉姆萨染色涂片被认为是最有效的诊断组合。
