Kawashima Hideki, Ohnishi Masao, Ogawa Satoshi
Bioscience Laboratory, Miyako College, Iwate Prefectural University, Japan.
J Oleo Sci. 2013;62(7):465-70. doi: 10.5650/jos.62.465.
In the present study, we show that four unusual cholesterol precursors, 5α-cholesta-8,14-dien-3β-ol, 4-methyl-5α-cholesta-8,24-dien-3β-ol (4-methylzymosterol), 4,4,14-trimethyl-5α-cholesta-8,24-dien-3β-ol (lanosterol), and 4,4-dimethyl-5α-cholesta-8,24-dien-3β-ol (4,4-dimethylzymosterol) are present in testes of the limpet Cellana grata, which is part of one of the most primitive gastropod families (Nacelllidae Family). The distribution of these sterols in testes and ovaries of four dominant species of limpets, Cellana grata, Cellana toreuma, Nipponacmea concinna, and Nipponacmea fuscoviridis, was examined by capillary gas chromatography-mass spectrometry. Based on the data, we discuss about the biological roles and possible application as bio-resources of these sterols. This is the first identification of 5α-cholesta-8, 14-dien-3β-ol, 4-methylzymosterol, and 4,4-dimethylzymosterol in marine invertebrate gonads.
在本研究中，我们发现四种不寻常的胆固醇前体，即5α-胆甾-8,14-二烯-3β-醇、4-甲基-5α-胆甾-8,24-二烯-3β-醇（4-甲基酵母甾醇）、4,4,14-三甲基-5α-胆甾-8,24-二烯-3β-醇（羊毛甾醇）和4,4-二甲基-5α-胆甾-8,24-二烯-3β-醇（4,4-二甲基酵母甾醇）存在于笠贝中华笠贝（Cellana grata）的睾丸中，中华笠贝属于最原始的腹足纲家族之一（笠贝科）。通过毛细管气相色谱-质谱联用技术检测了这四种甾醇在四种优势笠贝物种，即中华笠贝、托氏笠贝（Cellana toreuma）、扁平日本笠贝（Nipponacmea concinna）和褐绿日本笠贝（Nipponacmea fuscoviridis）的睾丸和卵巢中的分布情况。基于这些数据，我们讨论了这些甾醇的生物学作用以及作为生物资源的可能应用。这是首次在海洋无脊椎动物性腺中鉴定出5α-胆甾-8,14-二烯-3β-醇、4-甲基酵母甾醇和4,4-二甲基酵母甾醇。
