Sanchez Freddy M, Berges Bradford K
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA.
Methods Mol Biol. 2013;1031:215-22. doi: 10.1007/978-1-62703-481-4_24.
Engraftment of immunodeficient mice with a human immune system (humanized mice) provides a model system to study pathogens that target human immune cells. Humanized Rag2(-/-)γc(-/-) mice produce the major target cells of HIV-1 and these cells can be detected in primary and secondary lymphoid tissues, as well as in the vaginal and rectal mucosa and brain tissues. This humanized model has already yielded important findings on HIV-1 transmission, mechanisms of pathogenesis, and testing of novel antiviral strategies in vivo. Here, we describe the methods used to infect humanized mice with HIV-1 and to characterize plasma viral load and blood CD4(+) T cell depletion.
用人免疫系统对免疫缺陷小鼠进行移植（人源化小鼠），为研究靶向人类免疫细胞的病原体提供了一个模型系统。人源化Rag2(-/-)γc(-/-)小鼠产生HIV-1的主要靶细胞，这些细胞可在初级和次级淋巴组织以及阴道和直肠黏膜及脑组织中检测到。这种人源化模型已经在HIV-1传播、发病机制以及体内新型抗病毒策略的测试方面取得了重要发现。在这里，我们描述了用HIV-1感染人源化小鼠以及表征血浆病毒载量和血液CD4(+) T细胞耗竭的方法。
