牡荆叶乙酸乙酯提取物的抗丝虫活性的体外研究。

Antifilarial activity of ethyl acetate extract of Vitex negundo leaves in vitro.

机构信息

Department of Microbiology, Barkatullah University, Bhopal-462 026, M.P., India.

出版信息

Asian Pac J Trop Med. 2013 Sep;6(9):689-92. doi: 10.1016/S1995-7645(13)60119-4.

DOI:10.1016/S1995-7645(13)60119-4

PMID:23827144

Abstract

OBJECTIVE

To evaluate the possible antifilarial effect of ethyl acetate extract of Vitex negundo (Verbenaceae) leaves against Setaria cervi filarial parasite in vitro.

METHODS

In vitro screening was done by the method of motility inhibition and MTT reduction assay with concentrations of 0.03 to 1.00 mg/mL for 2 to 24 h incubation periods respectively, for possible antifilarial effect by comparing with control.

RESULTS

In motility assay, complete inhibition of motility was observed and in MTT reduction assay which gave >50% reduction for concentrations 0.20, 0.50 and 1.00 mg/mL at 10, 6 and 2 h incubation periods respectively in a dose dependent manner (P<0.05). An antifilarial effect imparted by plant extract was found to be a function of their relative concentrations. Inhibitory concentration (IC50) for the plant extract was found to be 0.16 mg/mL.

CONCLUSIONS

The present study recorded significant antifilarial effect of Vitex negundo plant extract and contributed toward the development of database for novel drug candidates for lymphatic filariasis.

摘要

目的

评估牡荆叶乙酸乙酯提取物（马鞭草科）对体外感染的小拟胸苷线虫的抗丝虫作用。

方法

采用体外筛选法，以 0.03 至 1.00 mg/mL 的浓度分别孵育 2 至 24 小时，通过与对照相比，评估可能的抗丝虫作用，通过运动抑制和 MTT 减少测定法进行。

结果

在运动试验中，观察到运动完全抑制，在 MTT 减少试验中，在 10、6 和 2 小时孵育期，浓度为 0.20、0.50 和 1.00 mg/mL 时，分别观察到>50%的减少，呈剂量依赖性（P<0.05）。植物提取物的抗丝虫作用被发现是其相对浓度的函数。植物提取物的抑制浓度（IC50）为 0.16 mg/mL。