评估Terminalia sp.叶提取物对牛丝状寄生虫Setaria cervi 的杀微丝蚴活性。
Assessment of macrofilaricidal activity of leaf extracts of Terminalia sp. against bovine filarial parasite Setaria cervi.
机构信息
Medicinal and Aromatic Plants, Regional Plant Resource Centre, Bhubaneswar, Odisha, India.
Medicinal and Aromatic Plants, Regional Plant Resource Centre, Bhubaneswar, Odisha, India.
出版信息
J Infect Public Health. 2018 Sep-Oct;11(5):643-647. doi: 10.1016/j.jiph.2018.01.006. Epub 2018 Feb 13.
Antifilarial potential of three medicinal plants namely, Terminalia bellerica, Terminalia chebula and Terminalia catappa was explored using Setaria cervi, a bovine filarial parasite at concentrations of 2.5, 5 and 10mg/ml. Amongst all the extracts, methanol extract of T. bellerica showed highest macrofilaricidal activity i.e. 84.63±1.11 at 10mg/ml in MTT reduction assay with IC value of 2.7mg/ml. which was better than the standard DEC i.e. 79.22±3.1% at 10mg/ml with IC value 2.84mg/ml. Other plant extracts showed mild in vitro macrofilaricidal activity. T. bellerica methanol extract exhibited significant GST activity of 18.86±0.21 and 12.83±0.03μM/ml/min at 5 and 10mg/ml with percentage inhibition value of 73.96% and 82.29% respectively. DEC showed GST activity value of 40.03±4.14 and 21.48±6.44μM/ml/min with percentage inhibition value of 21.76% and 58.01% at 5 and 10mg/ml respectively. Thus, methanol extract of leaves of T. bellerica exhibited highly significant antifilarial potential and needs detailed analysis.
三种药用植物，即诃子、使君子和榄仁，被用来探索它们对抗丝状寄生虫的潜力，所用寄生虫为牛丝状寄生虫——旋盘尾丝虫，浓度分别为 2.5、5 和 10mg/ml。在所有提取物中，诃子的甲醇提取物在 MTT 还原试验中显示出最高的杀微丝蚴活性，即在 10mg/ml 时为 84.63±1.11%，IC 值为 2.7mg/ml。这比标准 DEC（依维菌素）的杀微丝蚴活性更好，即在 10mg/ml 时为 79.22±3.1%，IC 值为 2.84mg/ml。其他植物提取物显示出轻度的体外杀微丝蚴活性。诃子甲醇提取物在 5 和 10mg/ml 时分别显示出 18.86±0.21 和 12.83±0.03μM/ml/min 的 GST 活性，抑制率分别为 73.96%和 82.29%。DEC 在 5 和 10mg/ml 时的 GST 活性值分别为 40.03±4.14 和 21.48±6.44μM/ml/min，抑制率分别为 21.76%和 58.01%。因此，诃子叶的甲醇提取物表现出很强的抗丝虫潜力，需要进一步分析。
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