筛选诱变剂提高雨生红球藻虾青素高产菌株的效率。

Efficiency of selected mutagens in generating Xanthophyllomyces dendrorhous strains hyperproducing astaxanthin.

机构信息

Institute of Technology of Plant Origin Food, Poznan University of Life Sciences, Poland.

出版信息

Pol J Microbiol. 2013;62(1):67-72.

Abstract

Astaxanthin is a xanthophill pigment with commercial application in the aquaculture, pharmaceutical, food and cosmetic industries. The red yeast Xanthophyllomyces dendrorhous is one of the most promising microorganisms for its industrial production. However, astaxanthin content in wild yeast strains is low. Pigment production by X. dendrorhous can be improved by mutagenesis. The aim of the study was to assess the efficiency of four mutagens: UV radiation, benomyl, ethyl methanesulfonate and ethidium bromide in generating asthaxanthin-hyperproducing strains of the yeast Xanthophyllomyces dendrorhous DSM 5626. Mutations with benomyl, ethidium bromide and UV radiation generated a group of hyperpigmented mutants exhibiting increases up to 100% in astaxanthin content. Ethyl methanesulfonate turned out to be useless in this respect.

摘要

虾青素是一种类胡萝卜素色素，在水产养殖、制药、食品和化妆品行业具有商业应用价值。红发夫酵母 Xanthophyllomyces dendrorhous 是最有前途的工业生产微生物之一。然而，野生酵母菌株中的虾青素含量较低。通过诱变可以提高 X. dendrorhous 的色素产量。本研究的目的是评估四种诱变剂：紫外线辐射、苯菌灵、乙基甲烷磺酸盐和溴化乙锭在产生虾青素高产酵母 Xanthophyllomyces dendrorhous DSM 5626 菌株方面的效率。苯菌灵、溴化乙锭和紫外线辐射的突变产生了一组色素加深的突变体，虾青素含量增加高达 100%。在这方面，乙基甲烷磺酸盐证明是无用的。