Department of Microbiology, Gdańsk University of Technology, Poland.
Pol J Microbiol. 2013;62(1):81-4.
Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.
快速、可靠的诊断是成功治疗感染的必要条件。此类诊断检测方法一直在不断发展。本文提出了一种基于 RAPD 模式比较的方法，用于选择基于 PCR 的诊断的分子靶标。选择了编码 Candida glabrata CBS138 假设蛋白的序列。从用 Candida glabrata 污染的血液样本中提取的 DNA 的 PCR 和实时 PCR 反应的检测限估计为 1 CFU/ml。因此，本研究中开发的检测方法似乎有望成为 C. glabrata 感染诊断的一种补充方法。
