Muller Héloïse, Thierry Agnès, Coppée Jean-Yves, Gouyette Catherine, Hennequin Christophe, Sismeiro Odile, Talla Emmanuel, Dujon Bernard, Fairhead Cécile
Institut Pasteur, Unité de Génétique Moléculaire des Levures, UPMC Univ Paris 06, CNRS URA 2171, 25 rue du Dr Roux, Paris, France.
Fungal Genet Biol. 2009 Mar;46(3):264-76. doi: 10.1016/j.fgb.2008.11.006. Epub 2008 Dec 6.
The genomic sequence of the type strain of the opportunist human pathogen Candida glabrata (CBS138, ATCC 2001) is available since 2004. This allows the analysis of genomic structure of other strains by comparative genomic hybridization. We present here the molecular analysis of a collection of 183 C. glabrata strains isolated from patients hospitalized in France and around the world. We show that the mechanisms of microevolution within this asexual species include rare reciprocal chromosomal translocations and recombination within tandem arrays of repeated genes, and that these account for the frequent size heterogeneity between chromosomes across strains. Gene tandems often encode cell wall proteins suggesting a possible role in adaptation to the environment.
自2004年起，机会性人类病原体光滑念珠菌（CBS138，ATCC 2001）模式菌株的基因组序列已可获取。这使得通过比较基因组杂交分析其他菌株的基因组结构成为可能。我们在此展示了对从法国及世界各地住院患者中分离出的183株光滑念珠菌菌株的分子分析。我们发现，这个无性物种内的微进化机制包括罕见的相互染色体易位以及重复基因串联阵列内的重组，而且这些机制导致了不同菌株间染色体大小的频繁异质性。基因串联通常编码细胞壁蛋白，这表明其在适应环境方面可能发挥作用。
