Ogino Mizuna, Matsuura Akihiro, Yamazaki Atusi, Irimajiri Mami, Suzuki Yoshihiro, Kushibiki Shiro, Singu Hroyuki, Kasuya Etsuko, Hasegawa Yoshihisa, Hodate Koichi
School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
Anim Sci J. 2014 Jan;85(1):58-68. doi: 10.1111/asj.12090. Epub 2013 Jul 5.
The secretion rhythms of plasma cortisol (CORT) and prolactin (PRL), hormones related to stress responsiveness and biological rhythm and controlled by light and temperature, were investigated under varying external environments and different management techniques. Serial blood samples were collected from female cattle reared in free-stall and freely fed (FF) conditions (n = 4) or in tie-stall and restricted feeding (RF) conditions (hay and concentrate twice daily, n = 4). Plasma CORT and PRL concentrations, eating behavior, and environmental parameters were analyzed. Cyclic patterns for each parameter were examined using spectral analysis, and correlations between CORT, PRL and other parameters were investigated using cross-spectral analysis. Under FF conditions, CORT secretion was not related to the lighting intensity and eating behavior. However, under RF conditions, the CORT secretion rhythm showed a distinct correlation with lighting intensity and eating behavior. Under FF conditions, the PRL secretion rhythm was similar in all seasons. However, under RF conditions, the PRL rhythm oscillated with high frequency in summer and low frequency in winter, indicating a seasonal change in rhythm. The present study demonstrates that hormone secretion rhythms change under different environments and management techniques.
在不同的外部环境和不同的管理技术条件下，研究了血浆皮质醇（CORT）和催乳素（PRL）的分泌节律，这两种激素与应激反应性和生物节律相关，并受光照和温度控制。从自由牛舍自由采食（FF）条件下饲养的母牛（n = 4）或栓系牛舍限制饲养（RF）条件下饲养的母牛（每天两次干草和精料，n = 4）采集系列血样。分析血浆CORT和PRL浓度、采食行为和环境参数。使用频谱分析检查每个参数的周期性模式，并使用互谱分析研究CORT、PRL与其他参数之间的相关性。在FF条件下，CORT分泌与光照强度和采食行为无关。然而在RF条件下，CORT分泌节律与光照强度和采食行为表现出明显的相关性。在FF条件下，PRL分泌节律在所有季节都相似。然而在RF条件下，PRL节律在夏季高频振荡，在冬季低频振荡，表明节律存在季节性变化。本研究表明，激素分泌节律在不同环境和管理技术下会发生变化。
