Johnston T C, Rucker E B, Cochrum L, Hruska K S, Vandegrift V
Department of Biological Sciences, Murray State University, KY 42071.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):407-15. doi: 10.1016/0006-291x(90)92106-a.
The luxA and luxB genes of bioluminescent bacteria encode the alpha and beta subunits of luciferase, respectively. Sequences of the luxA and luxB genes of Xenorhabdus luminescens, the only terrestrial bioluminescent bacterium known, were determined and the amino acid sequence of luciferase deduced. The alpha subunit was found to contain 360 amino acids and has a calculated molecular weight of 41,005 Da, while the beta subunit contains 327 amino acids and has a calculated molecular weight of 37,684 Da. Alignment of this luciferase with the luciferases of three marine bacteria showed 196 (or 55%) conserved residues in the alpha subunit and 114 (or 35%) conserved residues in the beta subunit. The highest degree of homology between any two species was between the luciferases of X. luminescens and Vibrio harveyi with 84% identity in the alpha subunits and 59% identity in the beta subunits.
发光细菌的luxA和luxB基因分别编码荧光素酶的α亚基和β亚基。已测定了已知的唯一陆生发光细菌——发光 Xenorhabdus 的luxA和luxB基因序列，并推导了荧光素酶的氨基酸序列。发现α亚基含有360个氨基酸，计算分子量为41,005道尔顿，而β亚基含有327个氨基酸，计算分子量为37,684道尔顿。将这种荧光素酶与三种海洋细菌的荧光素酶进行比对，结果显示α亚基中有196个（或55%）保守残基，β亚基中有114个（或35%）保守残基。任何两个物种之间同源性最高的是发光 Xenorhabdus 和哈维氏弧菌的荧光素酶，α亚基的同一性为84%，β亚基的同一性为59%。
