Goulian M, Grimm S L
Department of Medicine, University of California, San Diego, La Jolla 92093-0613.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):627-34. doi: 10.1016/0006-291x(90)92138-p.
Three DNA polymerases that use poly(rA).oligo(dT) were partially purified from cytoplasmic extracts of cultured mouse cells (after removal of mitochondria), and characterized. One is similar to, and may be the same as, the mitochondrial DNA polymerase gamma. The other two enzymes, one 7.5 S and the other 3.6 S, share some properties with DNA polymerases beta and gamma, e.g. their responses to certain inhibitors; however, they are not clearly identified with any previously well-characterized mammalian DNA polymerases. It is also demonstrated that the response of DNA polymerase gamma to N-ethylmaleimide is template dependent, and that DNA polymerase alpha has an authentic (albeit small) activity with poly(rA).oligo(dT).
从培养的小鼠细胞（去除线粒体后）的细胞质提取物中部分纯化出三种使用聚（rA）·寡聚（dT）的DNA聚合酶，并对其进行了表征。其中一种与线粒体DNA聚合酶γ相似，可能就是同一种酶。另外两种酶，一种为7.5 S，另一种为3.6 S，它们与DNA聚合酶β和γ有一些共同特性，例如它们对某些抑制剂的反应；然而，它们与任何先前已充分表征的哺乳动物DNA聚合酶都没有明确的对应关系。还证明了DNA聚合酶γ对N - 乙基马来酰亚胺的反应是模板依赖性的，并且DNA聚合酶α对聚（rA）·寡聚（dT）具有真实的（尽管很小）活性。
