Gaisbauer M, Schleich T, Stickl H A, Wilczek I
Institut für Toxikologie und Umwelthygiene der Technischen Universität München.
Arzneimittelforschung. 1990 May;40(5):594-8.
Chemiluminescence was used as an indicator for phagocytic activity of granulocytes induced by zymosan in whole blood. Luminol was used to amplify the luminescence measured. Methods and conditions of the trial were varied and the dependence on the methods applied became evident. The effects of echinacea-extract (Echinacin) and phorbolester (PMA) in various concentrations on phagocytic activity of whole blood heparinized with 10 I.E./ml heparin as well as on phagocytic activity of erytrocytolized blood were investigated. The reaction of the granulocytes--phagocytis and therefore chemilumenescence--under the influence of echinacea extract depends on the doses and methods applied. Therefore standardized methods and investigations of various immunoparameters as well as clinical investigations are necessary to prove the immunostimulative effect of socalled immunotherapeutics. This has not been undertaken up to now. Dose and method dependent single results cannot be a convincing justification for specific therapeutic medication.
化学发光被用作指示酵母聚糖诱导全血中粒细胞吞噬活性的指标。鲁米诺用于增强所测的发光。试验方法和条件各不相同，且对所应用方法的依赖性变得明显。研究了不同浓度的紫锥菊提取物（紫锥菊素）和佛波酯（PMA）对用10国际单位/毫升肝素抗凝的全血吞噬活性以及对红细胞溶解血液吞噬活性的影响。在紫锥菊提取物影响下粒细胞的反应——吞噬作用以及由此产生的化学发光——取决于所应用的剂量和方法。因此，需要标准化方法、对各种免疫参数的研究以及临床研究来证明所谓免疫治疗剂的免疫刺激作用。到目前为止尚未进行此类研究。依赖剂量和方法的单个结果不能成为特定治疗用药令人信服的理由。
