The Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115, Taiwan.
Analyst. 2013 Sep 7;138(17):4823-9. doi: 10.1039/c3an00653k. Epub 2013 Jul 5.
We developed the first dual-ion-trap mass analyzer which can detect ions with a high mass-to-charge ratio (m/z > 6000). The first ion trap is a quadrupole ion trap (QIT), which was operated by step scanning of the trapping frequency for a sample containing mixtures of biomolecules. The second ion trap, linear ion trap (LIT), was utilized to capture selected ions ejected out of the QIT so that all ions from the QIT can be examined one by one. It was found that the ions can be transferred from the QIT to the LIT with ~60% efficiency for large biomolecular ions with high m/z. This is by far the highest transfer efficiency in the dual ion trap device for high-mass ions.
我们开发了首个双离子阱质谱分析仪，可检测具有高质荷比（m/z>6000）的离子。第一个离子阱是四极离子阱（QIT），通过对含有生物分子混合物的样品的捕获频率进行分步扫描来操作。第二个离子阱，线性离子阱（LIT），用于捕获从 QIT 中射出的选定离子，以便逐个检查来自 QIT 的所有离子。结果发现，对于具有高 m/z 的大生物分子离子，从 QIT 到 LIT 的离子转移效率约为 60%。这是迄今为止用于高质量离子的双离子阱装置中的最高传输效率。
