[乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸对血管外膜成纤维细胞中血管紧张素II诱导的胶原合成的影响]

[Effects of Ac-SDKP on angiotensin II-induced collagen synthesis in vascular adventitial fibroblasts].

作者信息

Wang Ting, Kong Xiang-Quan, Wang Wei-Hua

机构信息

Department of Clinical Medicine, Wannan Medical College, Wuhu 241000, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2013 Mar;29(2):179-81, 192.

PMID:23833979

Abstract

OBJECTIVE

To investigate the effect of an anti-fibrotic tetra peptide Ac-SDKP on vascular fibrosis by regulating extracellular regulated protein kinase (ERK1/2) activity through Ang II.

METHODS

Rat vascular adventitial fibroblasts were cultured in vitro. They were randomly divided into control group, Ang II (10(-6) mmol/L) group, Ang II and Ac-SDKP joint action group, PD98059 group. Type I, III collagen contents in adventitia fibroblasts were measured by RT-PCR and the expressions of matrix metalloproteinases (MMP-2) and transforming growth factor-beta1 (TGF-beta1) were determined by Western blot.

RESULTS

Ac-SDKP could reduced Ang II-induced expression of type I, III collagen secretion and TGF-beta1 at mRNA,and increase MMP-2 expression, PD98059 could inhibit the above effect.

CONCLUSION

The results suggested that Ac-SDKP could inhibit the formation and development of vascular fibrosis through blocking ERK1/2 pathway mediated by Ang II. Ac-SDKP therefore served as an antifibrotic factor in vascular fibrosis.

摘要

目的

通过血管紧张素II（Ang II）调节细胞外调节蛋白激酶（ERK1/2）活性，探讨抗纤维化四肽Ac-SDKP对血管纤维化的影响。

方法

体外培养大鼠血管外膜成纤维细胞。将其随机分为对照组、Ang II（10(-6) mmol/L）组、Ang II与Ac-SDKP联合作用组、PD98059组。采用RT-PCR检测外膜成纤维细胞中I、III型胶原含量，Western blot检测基质金属蛋白酶（MMP-2）和转化生长因子-β1（TGF-β1）的表达。