Pourlis A F, Antonopoulos J
Laboratory of Anatomy, Histology & Embryology, School of Veterinary Medicine, University of Thessaly, P.O. Box 199, Karditsa, GR-43100, Greece.
Anat Histol Embryol. 2014 Aug;43(4):294-300. doi: 10.1111/ahe.12076. Epub 2013 Jul 9.
The onset of ossification centres of the pelvic girdle and leg skeleton of the quail in embryos and juvenile birds were studied. Specimens, which were cleared and were stained with Alcian Blue and Alizarin Red S, were examined at the stereomicroscope. The ilium and the pubis began to ossify at the 8th day (E8), whereas the ischium at E9. Perichondral ossification was observed at E6 in the femur, tibia and fibula. A secondary ossification centre was detected in the proximal epiphysis of the tibiotarsus at the 15th post-hatching day (P15). The patella began to ossify at P30. Regarding the tarsal bones tibiale, pre-tibiale and fibulare, ossification was observed at the E15, E12 and E16, respectively. The metatarsals II, III, IV ossified at E7, whereas the metatarsal I at E11. The centres of ossification of the 1st phalanges of all digits were observed at E9. At the same day, the ossification centres of the 2nd phalanx of digits II and III as well as the 3rd phalanx of digit III appeared. At E10, ossification was observed in the 2nd phalanx of digit I, in the 3rd phalanx of digit II and in the 2nd and 3rd phalanx of digit IV. In the 4th phalanx of digit III and in the terminal phalanges of digit IV, ossification was observed at E11. The data presented here provide useful baseline information on the normal sequential pattern of ossification in the pelvic girdle and leg skeleton in this species.
对鹌鹑胚胎和幼鸟骨盆带及腿部骨骼骨化中心的发生情况进行了研究。将经过透明处理并用阿尔辛蓝和茜素红S染色的标本在体视显微镜下检查。髂骨和耻骨在第8天（胚胎期第8天，E8）开始骨化，而坐骨在E9开始骨化。在胚胎期第6天（E6），股骨、胫骨和腓骨观察到软骨膜骨化。在孵化后第15天（P15），在胫跗骨近端骨骺发现了一个次级骨化中心。髌骨在P30开始骨化。关于跗骨中的胫跗骨、胫前骨和腓跗骨，分别在E15、E12和E16观察到骨化。第二、三、四跖骨在E7骨化，而第一跖骨在E11骨化。所有趾的第一指骨的骨化中心在E9观察到。同一天，第二和第三趾的第二指骨以及第三趾第三指骨的骨化中心出现。在E10，第一趾的第二指骨、第二趾的第三指骨以及第四趾的第二和第三指骨观察到骨化。在第三趾的第四指骨和第四趾的末节指骨，在E11观察到骨化。这里呈现的数据为该物种骨盆带和腿部骨骼正常骨化顺序模式提供了有用的基线信息。
