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二氧化碳和pH值对剑带双腔吸虫(吸虫纲)尾蚴附着反应的影响(作者译)

[Influence of CO2 and pH on the attachment response of the cercaria of Diplostomum spathaceum (trematoda) (author's transl)].

作者信息

Haas W

出版信息

Z Parasitenkd. 1975;46(1):53-60. doi: 10.1007/BF00383667.

Abstract
  1. The attachment of the cercaria to artificial substrates (offered via dialyzing membranes) in definite media was investigated under conditions of variable pH and [CO2]. 2. A decrease of the pH of the substrate releases only attachments in CO2 containing media and consequently acts via CO2 systems of the medium. 3. As effective components of CO2 systems, dissolved CO2 + H2CO3 are confirmed. 4. The sensitivity of the reaction on gradients of the CO2 partial pressure (in solution) could be established by offering substrates with lowered pH in CO2 containing media. Thus, by raising the CO2 partial pressure from ca. 0,04% to 0,15% maximal fixation rates were obtained (Fig. 3). 5. The carboanhydrase inhibitor acetazolamide, when added to the medium, had no direct influence on the CO2 receptors.
摘要
  1. 在可变pH值和[CO₂]条件下,研究了尾蚴在特定培养基中(通过透析膜提供)附着于人工基质的情况。2. 底物pH值降低仅在含CO₂的培养基中释放附着作用,因此是通过培养基的CO₂系统起作用。3. 确认溶解的CO₂ + H₂CO₃是CO₂系统的有效成分。4. 通过在含CO₂的培养基中提供pH值降低的底物,可以确定对(溶液中)CO₂分压梯度反应的敏感性。因此,将CO₂分压从约0.04%提高到0.15%时可获得最大固定率(图3)。5. 向培养基中添加碳酸酐酶抑制剂乙酰唑胺对CO₂受体没有直接影响。

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