Cancer Research Institute of Tomsk Scientific Center of Siberian Branch of Russian Academy of Medical Science, Tomsk, Russia; Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
Ultrason Sonochem. 2014 Jan;21(1):40-2. doi: 10.1016/j.ultsonch.2013.06.013. Epub 2013 Jun 27.
To determine the effect of pulsed ultrasound (US) on radiation-induced cell killing, U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% of duty factor at 0.3 W/cm(2) and pulsed at 1 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed 24 h after the treatments. The result showed significant enhancement of cell killing in the combined treatments. However, the ratio of apoptotic cells induced either by X-rays or US alone did not significantly change. These findings suggest that pulsed US can enhance the anticancer effect of X-irradiation due to US streaming under non-inertial cavitational condition. This combined treatment can potentially enhance the therapeutic effect of radiation therapy.
为了确定脉冲超声（US）对辐射诱导细胞杀伤的影响，将 U937 和 Molt-4 细胞系用 1.0 MHz US 以 50%的占空比在 0.3 W/cm(2)下照射，并在 0、0.5、2.5 和 5 Gy 的 X 射线照射后立即以 1 Hz 的频率进行脉冲照射。在处理后 24 小时进行细胞检测。结果表明，联合处理可显著增强细胞杀伤作用。然而，无论是 X 射线还是 US 单独诱导的凋亡细胞比例均无明显变化。这些发现表明，由于非惯性空化条件下的 US 流，脉冲 US 可以增强 X 射线照射的抗癌效果。这种联合治疗可能会增强放射治疗的治疗效果。
