蝎毒素肽 SPVII 促进辐射细胞增殖并增加 IL-3 受体的表达。

BACKGROUND

The previous investigation demonstrated the radioprotective efficacy of peptides isolated from the venom of Buthus Martti Karsch. In this study, the effect of isolated scorpion venom peptide II (SVPII) on irradiated M-NFS-60 cells and mouse bone marrow mononuclear cells (BM-MNCs) was observed. The AlamarBlue cell viability assay, a colony-forming unit (CFU) assay, flow cytometry (FCM), immunofluorescence, and Western blotting were used to evaluate cell proliferation, cell cycle progression, and the expression of the IL-3 receptor (IL-3R) protein in non-irradiated and irradiated cells.

RESULTS

Proliferation of irradiated M-NFS-60 cells was significantly accelerated by SPVII, and this effect was further enhanced by co-application of IL-3. Similarly, SPVII increased the number of BM-MNC CFUs and this proliferative effect was greater in the presence of SVPII plus IL-3. In addition, SPVII significantly altered cell cycle progression; SVPII enhanced the fraction of unirradiated M-NFS-60 cells in S phase and the fraction of irradiated M-NFS-60 cells arrested in G2/M. The expression of IL-3R protein by unirradiated M-NFS-60 cells was enhanced significantly by SVPII, and SVPII-induced IL-3R overexpression was 10-fold greater in irradiated M-NFS-60 cells.

CONCLUSIONS

These results indicated the hematopoietic growth factor (HGF)-like effects of SVPII on irradiated cells, possibly mediated by upregulation of IL-3R.

背景

先前的研究表明，从 Buthus Martti Karsch 毒液中分离得到的肽具有放射防护作用。在这项研究中，观察了分离的蝎毒液肽 II（SVPII）对辐照的 M-NFS-60 细胞和小鼠骨髓单核细胞（BM-MNC）的影响。使用 AlamarBlue 细胞活力测定法、集落形成单位（CFU）测定法、流式细胞术（FCM）、免疫荧光和 Western blot 评估了未辐照和辐照细胞的细胞增殖、细胞周期进程和白细胞介素 3 受体（IL-3R）蛋白的表达。

结果

SVPVII 显著加速了辐照的 M-NFS-60 细胞的增殖，而 IL-3 的共同应用则进一步增强了这种作用。同样，SVPVII 增加了 BM-MNC CFU 的数量，而 SVPII 加 IL-3 的存在则使这种增殖作用更大。此外，SVPVII 显著改变了细胞周期进程；SVPII 增加了未辐照的 M-NFS-60 细胞在 S 期的比例和辐照的 M-NFS-60 细胞在 G2/M 期的比例。SVPII 显著增强了未辐照的 M-NFS-60 细胞的 IL-3R 蛋白的表达，而 SVPII 诱导的 IL-3R 过表达在辐照的 M-NFS-60 细胞中增加了 10 倍。