Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
J Sep Sci. 2013 Sep;36(17):2888-900. doi: 10.1002/jssc.201300550. Epub 2013 Aug 2.
A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using the most widespread hexane- and dichloromethane-based mobile phases. The randomization of mono-acyl TG standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of TG isomers. The mobile phase composition has no influence on the general retention pattern, but significant differences in the retention order of double bond (DB) positional isomers in hexane and dichloromethane mobile phases are described and compared with the previous literature data. Saturated TGs with fatty acyl chain length from C7:0 to C22:0 are partially separated using the hexane mobile phase but not at all with the dichloromethane mobile phase. The hexane mobile phase enables at least partial resolution of TG regioisomers with up to seven DBs, while the resolution of only ALA/AAL and ALnA/AALn isomers is achieved with the dichloromethane mobile phase. The effect of temperature differs significantly depending on the mobile phase composition. Retention times of TGs increase with increasing temperature in the hexane mobile phase, while an opposite effect is observed for the dichloromethane mobile phase.
已在 10 至 40°C 温度范围内，用最常用的正己烷和二氯甲烷为流动相，对银离子高效液相色谱中异构体三酰基甘油（TGs）的保留行为进行了系统研究。采用单酰基 TG 标准物的随机化和甘油与脂肪酸的随机酯化作用来制备 TG 异构体混合物。流动相组成对一般保留模式没有影响，但在正己烷和二氯甲烷流动相中二烯键（DB）位置异构体的保留顺序存在显著差异，并与以前的文献数据进行了比较。用正己烷流动相可部分分离具有 C7:0 至 C22:0 长链脂肪酸的饱和 TG，但用二氯甲烷流动相则完全无法分离。正己烷流动相可至少部分分离多达七个 DB 的 TG 区域异构体，而二氯甲烷流动相则仅可分离 ALA/AAL 和 ALnA/AALn 异构体。温度的影响因流动相组成而异。在正己烷流动相，TG 的保留时间随温度升高而增加，而在二氯甲烷流动相则观察到相反的效果。
