Physikalisch-Chemisches Institut, Ruprecht-Karls-Universität, Im Neuenheimer Feld 229, 69120 Heidelberg, Germany.
J Biophotonics. 2014 Jan;7(1-2):126-34. doi: 10.1002/jbio.201300052. Epub 2013 Jul 9.
Chemical and structural composition of wood biomass is studied by label-free and chemically specific Coherent Anti-Stokes Raman Scattering (CARS) microscopy. A concept developed for assignment and semi-quantitative imaging of sample components; cellulose, hemicellulose, and lignin; by multiplex CARS microspectroscopy and subsequent data analysis is presented. Specific imaging without fluorescence backround is achieved an order of magnitude faster compared with conventional Raman microscopy. Laser polarization control yield information on molecular arrangement in wood fibers. Narrowband CARS excitation of single vibrations allows for three-dimensional volume imaging. Thus, CARS microscopy has potential as an important instrument for characterization of lignocellulosic materials.
采用无标记和化学特异性相干反斯托克斯拉曼散射（CARS）显微镜研究木材生物质的化学和结构组成。本文提出了一种通过多路复用 CARS 微光谱和后续数据分析来分配和半定量成像样品成分（纤维素、半纤维素和木质素）的概念。与传统的拉曼显微镜相比，该方法在实现特定成像而没有荧光背景方面快了一个数量级。激光偏振控制提供了有关木材纤维中分子排列的信息。窄带 CARS 激发单个振动允许进行三维体积成像。因此，CARS 显微镜作为木质纤维素材料特性分析的重要工具具有潜力。
