Takeda M, Maeda M, Tsuji A
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Biomed Chromatogr. 1990 May;4(3):119-22. doi: 10.1002/bmc.1130040309.
A chemiluminescence high performance liquid chromatographic method for the determination of corticosteroids and tetrahydrocorticosteroids has been developed. Corticosteroids and their metabolites extracted from urine samples were separated using an ODS column and a mixture of methanol + water + 0.01 M sodium acetate solution (70:30:5) as eluent. The eluent from the column was mixed with the chemiluminescent solution containing lucigenin and Triton X-100 and a 0.28 M KOH solution by pumps and monitored by a chemiluminescence detector. No interference was encountered and the method is both precise and reproducible.
已开发出一种用于测定皮质类固醇和四氢皮质类固醇的化学发光高效液相色谱法。从尿液样本中提取的皮质类固醇及其代谢物，使用ODS柱，并以甲醇+水+0.01M醋酸钠溶液（70:30:5）的混合物作为洗脱剂进行分离。柱流出物通过泵与含有光泽精和 Triton X - 100的化学发光溶液以及0.28M氢氧化钾溶液混合，并由化学发光检测器进行监测。未遇到干扰，该方法既精确又可重复。
