Chemistry Department, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
J Am Chem Soc. 2013 Jul 31;135(30):11175-80. doi: 10.1021/ja4038406. Epub 2013 Jul 19.
Herpes simplex viruses display hundreds of gD glycoproteins, and yet their neutralization requires tens of thousands of antibodies per virion, leading us to ask whether a wild-type virion with just a single free gD is still infective. By quantitative analysis of fluorescently labeled virus particles and virus neutralization assays, we show that entry of a wild-type HSV virion to a cell does indeed require just one or two of the approximately 300 gD glycoproteins to be left unbound by monoclonal antibody. This indicates that HSV entry is an extraordinarily efficient process, functioning at the level of single molecular complexes.
单纯疱疹病毒显示数百种 gD 糖蛋白，但其中和需要每个病毒粒子数千个抗体，这使我们不禁要问，只有一个游离 gD 的野生型病毒粒子是否仍然具有感染性。通过对荧光标记病毒颗粒的定量分析和病毒中和试验，我们表明，野生型单纯疱疹病毒进入细胞确实只需要大约 300 个 gD 糖蛋白中的一个或两个不被单克隆抗体结合。这表明单纯疱疹病毒进入是一个极其高效的过程，作用于单个分子复合物的水平。
