Department of Dermatology, Venereology, and Allergology, Ruhr-University Bochum, Gudrunstrasse 56, D-44791 Bochum, Germany.
Cytokine. 2013 Oct;64(1):35-8. doi: 10.1016/j.cyto.2013.06.311. Epub 2013 Jul 6.
The role of transforming growth factor-β1 (TGFβ1) and Smad signalling has not been established in psoriasis treatment. We aimed to investigate the effect of combined treatment with salt water soaks and ultraviolet radiation on the expression of TGFβ1/Smad signalling proteins in a psoriatic model. We studied mRNA expression (real-time RT-PCR) of TGFβ1, TGFβ receptor type I (TGFβRI), Smad2, Smad3, Smad4, Smad7, minichromosome maintenance protein 7, and involucrin in normal as well as psoriatic epidermis models (PEM) which were treated for three consecutive days with differently concentrated salt water solutions [(3% NaCl; 30% NaCl, 30% Dead Sea salt water (DSSW)] and subsequent narrowband ultraviolet B (NB-UVB). In PEM, TGFβ1 and Smad3 was significantly increased as compared to normal epidermis models. By contrast, TGFβRI mRNA was significantly decreased in PEM. Significant increase of mRNA levels of TGFβ1, TGFβRI, Smad2 and Smad3 was predominantly observed in non-irradiated and irradiated PEM pre-treated with 30% NaCl and/or DSSW which was paralleled by increase of involucrin mRNA. In PEM pre-treated with DSSW, TGFβRI, Smad2, Smad3, Smad4, and Smad7 mRNA was significantly higher in irradiated PEM when compared to non-irradiated samples. It has been shown that TGFβ1/Smad signalling is altered in a psoriatic model and may play a role in the mode of action of salt water soaks and NB-UVB phototherapy of psoriasis.
转化生长因子-β1（TGFβ1）和 Smad 信号通路在银屑病治疗中的作用尚未确定。我们旨在研究盐水浸泡和紫外线辐射联合治疗对银屑病模型中 TGFβ1/Smad 信号通路蛋白表达的影响。我们研究了正常和银屑病表皮模型（PEM）中 TGFβ1、TGFβ 受体 I（TGFβRI）、Smad2、Smad3、Smad4、Smad7、微小染色体维持蛋白 7 和内披蛋白的 mRNA 表达（实时 RT-PCR），这些模型连续三天分别用不同浓度的盐水溶液[（3%NaCl；30%NaCl、30%死海盐水（DSSW）]和随后的窄带紫外线 B（NB-UVB）进行处理。在 PEM 中，与正常表皮模型相比，TGFβ1 和 Smad3 显著增加。相比之下，PEM 中 TGFβRI 的 mRNA 显著减少。在未辐照和辐照的 PEM 中，用 30%NaCl 和/或 DSSW 预处理后，TGFβ1、TGFβRI、Smad2 和 Smad3 的 mRNA 水平显著增加，内披蛋白 mRNA 也随之增加。在用 DSSW 预处理的 PEM 中，与未辐照的样品相比，辐照的 PEM 中 TGFβRI、Smad2、Smad3、Smad4 和 Smad7 的 mRNA 明显更高。已经表明，TGFβ1/Smad 信号通路在银屑病模型中发生改变，并且可能在盐水浸泡和 NB-UVB 光疗治疗银屑病的作用模式中发挥作用。
