Tolliday Nicola
Chemical Biology Platform, The Broad Institute of Harvard University and MIT, Cambridge, Massachusetts.
Curr Protoc Chem Biol. 2010 Sep 1;2(3):153-61. doi: 10.1002/9780470559277.ch100045.
There are many assays available for high-throughput assessment of mammalian cell viability or cytotoxicity. Approaches include measurement of metabolic capacity, intracellular adenosine triphosphate (ATP) levels, induction of apoptosis, intracellular esterase and protease activity, and cellular membrane integrity. This unit provides an in-depth protocol for measurement of cellular ATP levels as a readout of mammalian cell viability, using the CellTiter-Glo assay from Promega Corporation. A comparison of the key parameters (sensitivity, speed, and cost) for this and other common high-throughput viability assays is also presented. Curr. Protoc. Chem. Biol. 2:153-161 © 2010 by John Wiley & Sons, Inc.
有许多方法可用于高通量评估哺乳动物细胞活力或细胞毒性。方法包括测量代谢能力、细胞内三磷酸腺苷（ATP）水平、凋亡诱导、细胞内酯酶和蛋白酶活性以及细胞膜完整性。本单元提供了一种深入的方案，用于使用Promega公司的CellTiter-Glo检测法测量细胞ATP水平，以此作为哺乳动物细胞活力的读数。还介绍了该检测法与其他常见高通量活力检测法的关键参数（灵敏度、速度和成本）的比较。《化学与生物学实验指南》第2卷：153 - 161页 2010年 约翰威立国际出版公司版权所有
