Brinkman C J, Otto A J, Kijlstra A, Breebaart A C
Department of Ophthalmology, University of Amsterdam, The Netherlands.
Curr Eye Res. 1990;9 Suppl:125-30. doi: 10.3109/02713689008999431.
A secondary immune response in the rabbit eye can be provoked by intravenous injection of the antigen, previously injected intravitreally. The inflammation process consists of iris hyperemia, pericorneal redness and cellular and proteinous exudates in the anterior chamber and vitreous. The influence of vitrectomy combined with lensectomy or preceded by extracapsular lens extraction on the character of the secondary immune response has been studied in the rabbit eye. Both eyes of adult rabbits were injected intravitreally with human serum albumin. Five to ten weeks later, when the primary inflammation process had vanished, the vitreous and the lens were removed from the right eye in one group of animals. In a second group of rabbits, an extracapsular lens extraction was performed upon both eyes, later followed by vitrectomy of the right eye. Four weeks later all animals received an intravenous booster injection with human serum albumin. The eyes which still contained the vitreous developed a secondary inflammation consisting predominantly of cells and fibrin clots in the anterior chamber however without flare. On the contrary, the eyes which underwent vitrectomy developed a flare in the anterior chamber with only a few cells detectable. Analysis of cells and protein in the anterior chamber and histological evaluation supported the clinically observed differences between both eyes. These results suggest that the persistence of the vitreous is associated with the reactivation of a secondary immune response. Vitrectomy combined with lensectomy or preceded by extracapsular lens extraction leads to increased passive transfer of proteinous material through the vessels.
通过静脉注射先前已玻璃体内注射过的抗原，可激发兔眼的二次免疫反应。炎症过程包括虹膜充血、角膜周围发红以及前房和玻璃体中的细胞及蛋白质渗出物。已在兔眼中研究了玻璃体切割联合晶状体切除或在囊外晶状体摘除之后进行玻璃体切割对二次免疫反应特征的影响。成年兔的双眼均玻璃体内注射人血清白蛋白。五至十周后，当原发性炎症过程消失时，一组动物的右眼玻璃体和晶状体被摘除。在第二组兔中，双眼均进行囊外晶状体摘除，随后右眼进行玻璃体切割。四周后，所有动物均接受人血清白蛋白静脉加强注射。仍保留玻璃体的眼睛出现二次炎症，主要表现为前房内有细胞和纤维蛋白凝块，但无房水闪辉。相反，接受玻璃体切割的眼睛前房出现房水闪辉，仅可检测到少量细胞。对前房内细胞和蛋白质的分析以及组织学评估支持了双眼临床观察到的差异。这些结果表明，玻璃体的持续存在与二次免疫反应的重新激活有关。玻璃体切割联合晶状体切除或在囊外晶状体摘除之后进行玻璃体切割会导致蛋白质物质通过血管的被动转运增加。
