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掌握氨。

Getting to grips with ammonium.

机构信息

is in the Department of Psychiatry and the Center for Molecular Recognition , Columbia University , New York , United States , and the Division of Molecular Therapeutics , New York State Psychiatric Institute , New York , United States

出版信息

Elife. 2013 Jul 2;2:e01029. doi: 10.7554/eLife.01029.

DOI:10.7554/eLife.01029
PMID:23840933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3699818/
Abstract

A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.

摘要

一种可以监测细胞外铵浓度的荧光传感器是通过使用融合的绿色荧光蛋白来检测铵转运蛋白构象变化而制成的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0cd/3699818/2055fa71e0ee/elife01029f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0cd/3699818/2055fa71e0ee/elife01029f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0cd/3699818/2055fa71e0ee/elife01029f001.jpg

相似文献

1
Getting to grips with ammonium.掌握氨。
Elife. 2013 Jul 2;2:e01029. doi: 10.7554/eLife.01029.
2
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本文引用的文献

1
Fluorescent sensors reporting the activity of ammonium transceptors in live cells.报告活细胞中铵转运体活性的荧光传感器。
Elife. 2013 Jul 2;2:e00800. doi: 10.7554/eLife.00800.
2
Common folds and transport mechanisms of secondary active transporters.常见的二级主动转运体的折叠和转运机制。
Annu Rev Biophys. 2013;42:51-72. doi: 10.1146/annurev-biophys-083012-130429.
3
Structural biology. (Pseudo-)symmetrical transport.结构生物学。（准）对称转运
Science. 2013 Jan 25;339(6118):399-401. doi: 10.1126/science.1228465.
4
High-resolution x-ray structure of human aquaporin 5.人水通道蛋白5的高分辨率X射线结构
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13327-32. doi: 10.1073/pnas.0801466105. Epub 2008 Sep 3.
5
The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters.拟南芥根中高亲和力铵吸收的组织依赖于AMT1型转运蛋白的空间排列和生化特性。
Plant Cell. 2007 Aug;19(8):2636-52. doi: 10.1105/tpc.107.052134. Epub 2007 Aug 10.
6
Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.嗜热栖热放线菌古菌铵转运蛋白Amt-1的晶体结构。
Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):14994-9. doi: 10.1073/pnas.0506254102. Epub 2005 Oct 7.
7
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.通过Amt/MEP/Rh进行氨转运的机制：AmtB在1.35埃时的结构
Science. 2004 Sep 10;305(5690):1587-94. doi: 10.1126/science.1101952.
8
Structure and mechanism of the lactose permease of Escherichia coli.大肠杆菌乳糖通透酶的结构与机制
Science. 2003 Aug 1;301(5633):610-5. doi: 10.1126/science.1088196.
9
Circularly permuted green fluorescent proteins engineered to sense Ca2+.经工程改造以检测钙离子的环状排列绿色荧光蛋白。
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3197-202. doi: 10.1073/pnas.051636098. Epub 2001 Mar 6.
10
Circular permutation and receptor insertion within green fluorescent proteins.绿色荧光蛋白中的环形排列与受体插入
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11241-6. doi: 10.1073/pnas.96.20.11241.