Clinic for Endocrinology, Diabetes and Metabolic Disorders, Clinical Centre of Serbia, Faculty of Medicine, University of Belgrade, Dr Subotica 13, 11000 Belgrade, Serbia.
Int J Endocrinol. 2013;2013:401609. doi: 10.1155/2013/401609. Epub 2013 Jun 13.
We analyzed (a) insulin sensitivity (IS) and (b) glutathione peroxidase (GSH-Px), glutathione reductase (GR), and superoxide dismutase (SOD) antioxidant enzyme activity in type 2 diabetic (T2D) patients with atherothrombotic infarction (ATI) (group A), lacunar infarction (LI) (B), or without stroke (C) and in nondiabetics with ATI (D), LI (E), or without stroke (F). ATI and LI were confirmed by brain imaging IS levels were determined by minimal model (Si index), and the enzyme activity by spectrophotometry. In T2D patients, Si was lower in A and B versus C (1.14 ± 0.58, 1.00 ± 0.26 versus 3.14 ± 0.62 min(-1)/mU/l × 10(4), P < 0.001) and in nondiabetics in D and E versus F (3.38 ± 0.77, 3.03 ± 0.72 versus 6.03 ± 1.69 min(-1)/mU/l × 10(4), P < 0.001). Also, GSH-Px and GR activities were lower in A and B versus C (GSH-Px: 21.96 ± 3.56, 22.51 ± 1.23 versus 25.12 ± 1.67; GR: 44.37 ± 3.58, 43.50 ± 2.39 versus 48.58 ± 3.67 U/gHb; P < 0.001) and in D and E versus F (GSH-Px: 24.75 ± 3.02, 25.57 ± 1.92 versus 28.56 ± 3.91; GR: 48.27 ± 6.81, 49.17 ± 6.24 versus 53.67 ± 3.96 U/gHb; P < 0.001). Decreases in Si and GR were significantly related to both ATI and LI in T2D. Our results showed that decreased IS and impaired antioxidant enzymes activity influence ischemic stroke subtypes in T2D. The influence of insulin resistance might be exerted on the level of glutathione-dependent antioxidant enzymes.
我们分析了（a）胰岛素敏感性（IS）和（b）谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）抗氧化酶活性，这些活性存在于 2 型糖尿病（T2D）伴动脉血栓形成性梗死（ATI）（A 组）、腔隙性梗死（LI）（B 组）或无卒中（C 组）的患者，以及非糖尿病伴 ATI（D 组）、LI（E 组）或无卒中（F 组）的患者中。通过脑成像证实了 ATI 和 LI，IS 水平通过最小模型（Si 指数）确定，酶活性通过分光光度法确定。在 T2D 患者中，A 组和 B 组的 Si 低于 C 组（1.14±0.58、1.00±0.26 与 3.14±0.62 min(-1)/mU/l×10(4)，P<0.001），在非糖尿病患者中，D 组和 E 组的 Si 低于 F 组（3.38±0.77、3.03±0.72 与 6.03±1.69 min(-1)/mU/l×10(4)，P<0.001）。此外，A 组和 B 组的 GSH-Px 和 GR 活性低于 C 组（GSH-Px：21.96±3.56、22.51±1.23 与 25.12±1.67；GR：44.37±3.58、43.50±2.39 与 48.58±3.67 U/gHb；P<0.001），D 组和 E 组的 GSH-Px 和 GR 活性低于 F 组（GSH-Px：24.75±3.02、25.57±1.92 与 28.56±3.91；GR：48.27±6.81、49.17±6.24 与 53.67±3.96 U/gHb；P<0.001）。Si 和 GR 的降低与 T2D 患者的 ATI 和 LI 均显著相关。我们的结果表明，胰岛素敏感性降低和抗氧化酶活性受损影响 T2D 患者的缺血性脑卒中亚型。胰岛素抵抗的影响可能表现在谷胱甘肽依赖的抗氧化酶水平上。
