贝尔格莱德（b/b）实验大鼠某些组织中抗氧化防御酶的活性和谷胱甘肽含量
Activity of antioxidant defense enzymes and glutathione content in some tissues of the Belgrade (b/b) laboratory rat.
作者信息
Saicić Z S, Simović M O, Korać B, Blagojević D, Buzadzić B, Spasić M B, Petrović V M
机构信息
Department of Physiology, Institute for Biological Research Sinisa Stanković, Serbia, Yugoslavia.
出版信息
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1997 Jun;117(2):173-9. doi: 10.1016/s0742-8413(97)89892-1.
The activity of antioxidant defense (AD) enzymes--superoxide dismutase (SOD, EC 1.15.1.1.), catalase (CAT, EC 1.11.1.6.), glutathione peroxidase (GSH-Px, EC 1.11.1.9.), glutathione-S-transferase (GST, EC 2.5.1.18), glutathione reductase (GR, EC 1.6.4.2) and glutathione (GSH) content of the anemic Belgrade (b/b) laboratory rats--were measured and analyzed in liver, spleen, lung, heart, brain and testes in comparison with nonanemic controls. The activities of hepatic Mn SOD, CAT, GSH-Px and GST (P < 0.02, P < 0.01 and P < 0.005) were decreased in anemic, comparing with nonanemic animals, whereas the spleen CuZn SOD, Mn SOD, CAT and GSH-Px (P < 0.005, P < 0.02, P < 0.005 and P < 0.01) activities were increased. In the lung of anemic rats, Mn SOD, GSH-Px and GR (P < 0.005, P < 0.01, P < 0.05) activities were higher, whereas GST (P < 0.01) activity was lower in relation to nonanemic ones. In anemic rats, heart Mn SOD (P < 0.05) activity was increased, brain GSH-Px (P < 0.005) activity was lower, whereas GR (P < 0.02) activity was higher compared with nonanemic controls. CuZn SOD (P < 0.05) activity in the testes was elevated and GSH-Px (P < 0.05) reduced in anemic animals. GSH content was decreased in the liver (P < 0.01), lung and brain (P < 0.005) and increased in the spleen (P < 0.02) of anemic rats in relation to the controls. Our data suggest phenotype specific differences in the AD system of the Belgrade (b/b) rat tissues in comparison with nonanemic controls.
测定并分析了贫血的贝尔格莱德（b/b）实验大鼠肝脏、脾脏、肺、心脏、大脑和睾丸中抗氧化防御（AD）酶——超氧化物歧化酶（SOD，EC 1.15.1.1）、过氧化氢酶（CAT，EC 1.11.1.6）、谷胱甘肽过氧化物酶（GSH-Px，EC 1.11.1.9）、谷胱甘肽-S-转移酶（GST，EC 2.5.1.18）、谷胱甘肽还原酶（GR，EC 1.6.4.2）的活性以及谷胱甘肽（GSH）含量，并与非贫血对照组进行比较。与非贫血动物相比，贫血大鼠肝脏中的锰超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶活性降低（P<0.02、P<0.01和P<0.005），而脾脏中的铜锌超氧化物歧化酶、锰超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性升高（P<0.005、P<0.02、P<0.005和P<0.01）。与非贫血大鼠相比，贫血大鼠肺中的锰超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性较高（P<0.005、P<0.01、P<0.05），而谷胱甘肽-S-转移酶活性较低（P<0.01）。与非贫血对照组相比，贫血大鼠心脏中的锰超氧化物歧化酶活性升高（P<0.05），大脑中的谷胱甘肽过氧化物酶活性降低（P<0.005），而谷胱甘肽还原酶活性较高（P<0.02）。贫血动物睾丸中的铜锌超氧化物歧化酶活性升高（P<0.05），谷胱甘肽过氧化物酶活性降低（P<0.05）。与对照组相比，贫血大鼠肝脏中的谷胱甘肽含量降低（P<0.01），肺和大脑中的谷胱甘肽含量降低（P<0.005），脾脏中的谷胱甘肽含量升高（P<0.02）。我们的数据表明，与非贫血对照组相比，贝尔格莱德（b/b）大鼠组织的抗氧化防御系统存在表型特异性差异。
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