Xiong Qi, Zhang Li-Cheng, Zhang Li-Hai, Yao Qi, Tang Peifu
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing, China.
Zhongguo Gu Shang. 2013 Apr;26(4):324-7.
To compare the effect of recombinant OPG-Fc and recombinant RANK protein on the differentiation of osteoclast precursors.
Mouse osteoblasts cell lines were incubated with osteoclast precursors cell lines RAW 264.7 for 9 days with 10(-5) g/L rhRANK or rhOPG-Fc or PBS added to the coculture system. TRAP stain positive cells counting and cortical bone pit formation counting were performed in the 9th day.
Multinuleated TRAP stain positive cells were observed in the cocluture systems after 6 days incubation,and plenty of mature osteoclasts could be observed in the 9th day. With the addition of 10(-5) g/L rhOPG-Fc or rhRANK, multinucleated giant cells and cortical bone pit formation couting decreased significantly compared with the control group, and the rhRANK group decreased more significantly than the rhOPG-Fc group.
Both rhOPG-Fc and rhRANK can inhabit the differentiation of osteoclast precursors and prevent them forming mature osteoclasts,moreover,the rhRANK shows the significant inhabition effect than the rhOPG-Fc.
比较重组人骨保护素-Fc（OPG-Fc）和重组核因子κB受体活化因子（RANK）蛋白对破骨细胞前体细胞分化的影响。
将小鼠成骨细胞系与破骨细胞前体细胞系RAW 264.7共培养9天，共培养体系中加入10⁻⁵ g/L重组人RANK（rhRANK）或重组人骨保护素-Fc（rhOPG-Fc）或磷酸盐缓冲液（PBS）。在第9天进行抗酒石酸酸性磷酸酶（TRAP）染色阳性细胞计数和皮质骨陷窝形成计数。
共培养体系孵育6天后可见多核TRAP染色阳性细胞，第9天可见大量成熟破骨细胞。加入10⁻⁵ g/L rhOPG-Fc或rhRANK后，多核巨细胞和皮质骨陷窝形成计数较对照组显著降低，且rhRANK组降低更显著。
rhOPG-Fc和rhRANK均可抑制破骨细胞前体细胞的分化，阻止其形成成熟破骨细胞，且rhRANK的抑制作用比rhOPG-Fc更显著。
