Schaeffer W I, Simkins S G, Lamore B J
University of Vermont, Department of Microbiology, Burlington 05405.
In Vitro Cell Dev Biol. 1990 Jul;26(7):737-40. doi: 10.1007/BF02624431.
Populations of normal and tumorigenic epithelial cells were plated in normal (serum supplemented) and a minimal defined medium. The ability of cells to grow in the minimal defined medium vs. the normal medium was related to their tumorigenic ability. All of the clones that were capable of growth in the minimal defined medium demonstrated tumorigenic ability. None of the normal clones survived in the minimal defined medium. As expected, some of the tumorigenic clones could not grow in the minimal medium. However, at no time did a clone that grew in the minimal defined medium fail to demonstrate tumorigenic ability. This validated that this system can be an animal-free means for selecting tumorigenic epithelial cells out of a mixed population.
将正常上皮细胞和致瘤性上皮细胞群体接种于正常（添加血清）培养基和最低限度限定培养基中。细胞在最低限度限定培养基与正常培养基中生长的能力与其致瘤能力相关。所有能够在最低限度限定培养基中生长的克隆均表现出致瘤能力。正常克隆在最低限度限定培养基中无一存活。正如预期的那样，一些致瘤性克隆在最低限度培养基中无法生长。然而，在最低限度限定培养基中生长的克隆从未未能表现出致瘤能力。这证实了该系统可作为一种无动物的方法，从混合群体中筛选致瘤性上皮细胞。
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