Goodwin R H
USDA/ARS, Montana State University, Bozeman 59717.
In Vitro Cell Dev Biol. 1991 Jun;27A(6):470-8. doi: 10.1007/BF02631147.
Culture medium supplementation with vertebrate serum results in the selection of fibroblastoid insect cell lines and a general decline during continuous subculturing of both morphologic and functional differentiation of the surviving cells. Essential lipid mixtures can substitute for vertebrate serum in the culture of insect and some vertebrate cells, tissues, parasites, and pathogens. The provision of sterols and essential (with nonessential) polyunsaturated fatty acids as phospholipids in oxidation-protected peptoliposomes or proteoliposomes allows cells in culture to duplicate in vivo specific membranes more accurately. Such lipid-corrected membranes allow cultured cells to communicate with neighboring cells through the extracellular matrix, effectively transmit hormonal signals directly and via receptor control, and respond with various tissue-specific functions and differentiation states as directed.
用脊椎动物血清补充培养基会导致成纤维细胞样昆虫细胞系的选择，并且在存活细胞的连续传代培养过程中，其形态和功能分化普遍下降。必需脂质混合物可替代脊椎动物血清用于昆虫以及一些脊椎动物细胞、组织、寄生虫和病原体的培养。以氧化保护的肽脂质体或蛋白脂质体形式提供固醇和必需（以及非必需）多不饱和脂肪酸作为磷脂，可使培养中的细胞更准确地复制体内特定膜。这种脂质校正的膜使培养细胞能够通过细胞外基质与相邻细胞进行通讯，直接并通过受体控制有效地传递激素信号，并根据指示以各种组织特异性功能和分化状态做出反应。
