Ban Yanli, Chang Yongchun, Dong Baihua, Kong Beihua, Qu Xun
Department of Obstetrics and Gynaecology, Qilu Hospital of Shandong University, Ji'nan, Shandong Province, China.
J Int Med Res. 2013 Aug;41(4):1135-49. doi: 10.1177/0300060513487642. Epub 2013 Jul 11.
To localize indoleamine 2,3-dioxygenase (IDO) mRNA and protein and to undertake a functional study at the first trimester fetal-maternal interface in order to determine whether the distribution and function of IDO are related to recurrent spontaneous abortion (RSA).
Women undergoing legal pregnancy termination and women with RSA participated in this prospective study. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction were used to analyse levels of IDO protein and mRNA in placenta, decidua and HTR-8/SVneo cells. Culture medium collected from trophoblast villous explant or HTR-8/SVneo cell cultures was used to measure IDO activity in response to interferon (IFN)-γ treatment.
A total of 40 healthy women and 26 women with RSA provided samples of placenta and decidua. For normal pregnancies, IDO protein and mRNA was identified in placental trophoblasts, invasive extravillous trophoblasts and decidual glandular epithelium. IFN-γ significantly increased IDO activity in trophoblast villous explants and HTR-8/SVneo cells. Levels of IDO protein and mRNA in the placenta and decidua from normal pregnancies were significantly higher than in those from RSA.
Decreased levels of IDO protein and mRNA in the placenta and decidua from RSA suggest an important role for IDO in the maintenance of normal pregnancy.
定位吲哚胺2,3-双加氧酶（IDO）的mRNA和蛋白，并在孕早期母胎界面进行功能研究，以确定IDO的分布和功能是否与复发性自然流产（RSA）相关。
接受合法终止妊娠的妇女和患有RSA的妇女参与了这项前瞻性研究。采用免疫组织化学和实时逆转录-聚合酶链反应分析胎盘、蜕膜和HTR-8/SVneo细胞中IDO蛋白和mRNA的水平。从滋养层绒毛外植体或HTR-8/SVneo细胞培养物中收集的培养基用于测量对干扰素（IFN）-γ处理的IDO活性。
共有40名健康妇女和26名患有RSA的妇女提供了胎盘和蜕膜样本。对于正常妊娠，在胎盘滋养层细胞、侵入性绒毛外滋养层细胞和蜕膜腺上皮中鉴定出IDO蛋白和mRNA。IFN-γ显著增加了滋养层绒毛外植体和HTR-8/SVneo细胞中的IDO活性。正常妊娠胎盘和蜕膜中IDO蛋白和mRNA的水平显著高于RSA患者。
RSA患者胎盘和蜕膜中IDO蛋白和mRNA水平降低，提示IDO在维持正常妊娠中起重要作用。
