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八氢吲哚羧酸酰基化 ghrelin 在 ghrelin 产生细胞中细胞内分布的改变。

Changes in Subcellular Distribution of n-Octanoyl or n-Decanoyl Ghrelin in Ghrelin-Producing Cells.

机构信息

Department of Physiology, School of Medicine, Kurume University , Kurume , Japan.

出版信息

Front Endocrinol (Lausanne). 2013 Jul 9;4:84. doi: 10.3389/fendo.2013.00084. eCollection 2013.

DOI:10.3389/fendo.2013.00084
PMID:23847595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3705199/
Abstract

BACKGROUND

The enzyme ghrelin O-acyltransferase (GOAT) catalyzes the acylation of ghrelin. The molecular form of GOAT, together with its reaction in vitro, has been reported previously. However, the subcellular processes governing the acylation of ghrelin remain to be elucidated.

METHODS

Double immunoelectron microscopy was used to examine changes in the relative proportions of secretory granules containing n-octanoyl ghrelin (C8-ghrelin) or n-decanoyl ghrelin (C10-ghrelin) in ghrelin-producing cells of mouse stomachs. The dynamics of C8-type (possessing C8-ghrelin exclusively), C10-type (possessing C10-ghrelin only), and mixed-type secretory granules (possessing both C8- and C10-ghrelin) were investigated after fasting for 48 h or after 2 weeks feeding with chow containing glyceryl-tri-octanoate (C8-MCT) or glyceryl-tri-decanoate (C10-MCT). The dynamics of C8- or C10-ghrelin-immunoreactivity (ir-C8- or ir-C10-ghrelin) within the mixed-type granules were also investigated.

RESULTS

Immunoelectron microscopic analysis revealed the co-existence of C8- and C10-ghrelin within the same secretory granules (mixed-type) in ghrelin-producing cells. Compared to control mice fed standard chow, the ratio of C10-type secretory granules increased significantly after ingestion of C10-MCT, whereas that of C8-type granules declined significantly under the same treatment. After ingestion of C8-MCT, the proportion of C8-type secretory granules increased significantly. Within the mixed-type granules the ratio of ir-C10-ghrelin increased significantly and that of ir-C8-ghrelin decreased significantly upon fasting.

CONCLUSION

These findings confirmed that C10-ghrelin, another acyl-form of active ghrelin, is stored within the same secretory granules as C8-ghrelin, and suggested that the types of medium-chain acyl-molecules surrounding and available to the ghrelin-GOAT system may affect the physiological processes of ghrelin acylation.

摘要

背景

酶 ghrelin O-酰基转移酶 (GOAT) 催化 ghrelin 的酰化。GOAT 的分子形式及其体外反应先前已有报道。然而，调控 ghrelin 酰化的亚细胞过程仍有待阐明。

方法

采用双免疫电镜检查禁食 48 小时或用含有甘油三辛酸酯 (C8-MCT) 或甘油三癸酸酯 (C10-MCT) 的标准饲料喂养 2 周后，胃 ghrelin 产生细胞中含有 n-辛酰基 ghrelin (C8-ghrelin) 或 n-癸酰基 ghrelin (C10-ghrelin) 的分泌颗粒的相对比例的变化。研究了 C8 型（仅含有 C8-ghrelin）、C10 型（仅含有 C10-ghrelin）和混合型（同时含有 C8-和 C10-ghrelin）分泌颗粒的动力学变化。还研究了混合型颗粒内 ir-C8-或 ir-C10-ghrelin 的动力学变化。

结果

免疫电镜分析显示，ghrelin 产生细胞中的同一分泌颗粒（混合型）内共存 C8-和 C10-ghrelin。与标准饲料喂养的对照小鼠相比，摄入 C10-MCT 后 C10 型分泌颗粒的比例显著增加，而在相同处理下 C8 型颗粒的比例显著下降。摄入 C8-MCT 后，C8 型分泌颗粒的比例显著增加。在混合型颗粒内，ir-C10-ghrelin 的比例显著增加，ir-C8-ghrelin 的比例显著下降。

结论

这些发现证实了另一种活性 ghrelin 的酰基形式 C10-ghrelin 与 C8-ghrelin 储存在同一分泌颗粒内，并提示围绕 ghrelin-GOAT 系统并可用于该系统的中链酰基分子的类型可能影响 ghrelin 酰化的生理过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/bf082a597068/fendo-04-00084-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/73136ae60e5c/fendo-04-00084-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/31394b33ef97/fendo-04-00084-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/bf082a597068/fendo-04-00084-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/73136ae60e5c/fendo-04-00084-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/31394b33ef97/fendo-04-00084-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/396b/3705199/bf082a597068/fendo-04-00084-g003.jpg

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