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用于即时护理临床应用的集成荧光相关光谱装置。

Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications.

作者信息

Olson Eben, Torres Richard, Levene Michael J

机构信息

Department of Biomedical Engineering, Yale School of Engineering and Applied Science, New Haven, CT 06510, USA.

出版信息

Biomed Opt Express. 2013 Jun 11;4(7):1074-82. doi: 10.1364/BOE.4.001074. Print 2013 Jul 1.

DOI:10.1364/BOE.4.001074
PMID:23847733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3704089/
Abstract

We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the technique for clinical use. Integration of the focusing optics and sample chamber into a plastic component produces a design which is simple to align and operate. We validate the system by measurements on fluorescent dye, and compare the results to a commercial instrument. In addition, we demonstrate its application to measurements of concentration and multimerization of the clinically relevant protein von Willebrand factor (vWF) in human plasma.

摘要

我们描述了一种光学系统，该系统降低了荧光相关光谱法（FCS）的成本和复杂性，旨在提高该技术在临床应用中的适用性。将聚焦光学器件和样品腔集成到一个塑料部件中，产生了一种易于对准和操作的设计。我们通过对荧光染料的测量来验证该系统，并将结果与商用仪器进行比较。此外，我们展示了其在测量人血浆中临床相关蛋白血管性血友病因子（vWF）的浓度和多聚化方面的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/21130b0ce818/boe-4-7-1074-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/3a281fd3e683/boe-4-7-1074-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/95b3b1e392a8/boe-4-7-1074-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/917c465f3169/boe-4-7-1074-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/f6d966ca115f/boe-4-7-1074-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/21130b0ce818/boe-4-7-1074-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/3a281fd3e683/boe-4-7-1074-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/95b3b1e392a8/boe-4-7-1074-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/917c465f3169/boe-4-7-1074-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/f6d966ca115f/boe-4-7-1074-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67b4/3704089/21130b0ce818/boe-4-7-1074-g005.jpg

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本文引用的文献

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Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages.有证据表明，存在一道屏障阻止了磷脂酰肌醇 4,5-二磷酸从巨噬细胞正在形成的吞噬体中扩散出去。
Mol Biol Cell. 2011 Sep;22(18):3498-507. doi: 10.1091/mbc.E11-02-0114. Epub 2011 Jul 27.
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The use of fluorescence correlation spectroscopy (FCS) as an alternative biomarker detection technique: a preliminary study.
荧光相关光谱（FCS）作为替代生物标志物检测技术的应用：初步研究。
J Cell Mol Med. 2011 Dec;15(12):2706-11. doi: 10.1111/j.1582-4934.2011.01272.x.
4
Optical-fiber-microsphere for remote fluorescence correlation spectroscopy.用于远程荧光相关光谱学的光纤微球
Opt Express. 2009 Oct 12;17(21):19085-92. doi: 10.1364/OE.17.019085.
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Disposable microscope objective lenses for fluorescence correlation spectroscopy using latex microspheres.用于使用乳胶微球的荧光相关光谱分析的一次性显微镜物镜
Anal Chem. 2008 Sep 1;80(17):6800-4. doi: 10.1021/ac801016z. Epub 2008 Aug 6.
6
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