Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Aug;116(2):221-31. doi: 10.1016/j.oooo.2013.05.006.
This study investigates the oncogenic role of WWP1, an ubiquitin ligase linked to tumor promotion, in oral cancer.
An array-based comparative genomic hybridization was used to detect chromosomal changes in 10 oral cancer specimens. An additional 59 specimens and 6 cultured oral cancer cells were further examined to evaluate changes in the DNA copy number and messenger RNA (mRNA) and protein expression of WWP1.
The copy number of the WWP1 gene and its mRNA levels were significantly increased in the oral cancer specimens. An elevated WWP1 gene expression was observed in 6 cultured oral cancer cell lines. Knockdown of the endogenous WWP1 using small hairpin RNA further showed that deficiency of WWP1 suppressed cell growth and caused apoptosis in oral cancer cells.
Our results reveal that WWP1 might play an oncogenic role in oral cancer cells.
本研究旨在探讨与肿瘤促进相关的泛素连接酶 WWP1 在口腔癌中的致癌作用。
采用基于阵列的比较基因组杂交技术检测 10 例口腔癌标本中的染色体变化。进一步对另外 59 例标本和 6 株培养的口腔癌细胞进行检测，以评估 WWP1 的 DNA 拷贝数和信使 RNA（mRNA）和蛋白质表达的变化。
在口腔癌标本中，WWP1 基因的拷贝数及其 mRNA 水平显著增加。在 6 株培养的口腔癌细胞系中观察到 WWP1 基因表达升高。使用短发夹 RNA 敲低内源性 WWP1 进一步表明，WWP1 缺失抑制了口腔癌细胞的生长并诱导其凋亡。
我们的结果表明，WWP1 可能在口腔癌细胞中发挥致癌作用。
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