Biology of the Testis, Research Laboratory for Embryology and Genetics, Vrije Universiteit Brussel, Brussels, Belgium.
Fertil Steril. 2013 Oct;100(4):981-8. doi: 10.1016/j.fertnstert.2013.06.016. Epub 2013 Jul 11.
To translate spermatogonial stem cell (SSC) transplantation towards a clinical application.
Mouse green fluorescent protein (GFP)-positive testicular cells were labeled with (99m)technetium and microbubbles. These labeled cells were injected into the rete testis of isolated human testes under ultrasound guidance. Three different conditions were tested: 1) 800 μL of a 20 million cells/mL suspension; 2) 800 μL of a 10 million cells/mL suspension; and 3) 1,400 μL of a 10 million cells/mL suspension. After injection, the human cadaver testes were analyzed with the use of single-photon-emission computerized tomography (SPECT) imaging and histology.
Laboratory research environment.
PATIENT(S): Cadaver testes, obtained from autopsies at the pathology department.
INTERVENTION(S): Ultrasound-guided injection of mouse GFP-positive testicular cells.
MAIN OUTCOME MEASURE(S): Presence of radioactive-labeled cells in the human cadaver testes and GFP-positive cells in the seminiferous tubules.
RESULT(S): In all of the experimental groups, GFP-positive cells were observed in the seminiferous tubules, near and far from the rete testis, but also in the interstitium. On SPECT, significant difference was seen between the group injected with 800 μL of a 20 million cells/mL suspension (1,654.6 ± 907.6 mm³) and the group injected with 1,400 μL of a 10 million cells/mL suspension (3,614.9 ± 723.1 mm³). No significant difference was reached in the group injected with 800 μL of a 10 million cells/mL suspension.
CONCLUSION(S): Injecting cells in the human cadaver testis is feasible, but further optimization is required.
将精原干细胞（SSC）移植推向临床应用。
用（99m）锝和微泡标记小鼠绿色荧光蛋白（GFP）阳性睾丸细胞。这些标记的细胞在超声引导下被注入离体人睾丸的输出小管中。测试了三种不同的情况：1）2000 万细胞/mL 悬浮液 800μL；2）1000 万细胞/mL 悬浮液 800μL；3）1000 万细胞/mL 悬浮液 1400μL。注射后，使用单光子发射计算机断层扫描（SPECT）成像和组织学分析人尸睾丸。
实验室研究环境。
尸体睾丸，从病理科尸检中获得。
超声引导注射小鼠 GFP 阳性睾丸细胞。
放射性标记细胞在人尸睾丸中的存在和 GFP 阳性细胞在生精小管中的存在。
在所有实验组中，在靠近和远离输出小管的生精小管中以及在间质中都观察到 GFP 阳性细胞。在 SPECT 上，注射 800μL 2000 万细胞/mL 悬浮液的组（1654.6±907.6mm³）与注射 1400μL 1000 万细胞/mL 悬浮液的组（3614.9±723.1mm³）之间存在显著差异。在注射 800μL 1000 万细胞/mL 悬浮液的组中未达到显著差异。
向人尸睾丸中注射细胞是可行的，但需要进一步优化。
