The Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033 Oslo, Norway.
Toxicol In Vitro. 2013 Sep;27(6):1962-9. doi: 10.1016/j.tiv.2013.06.012. Epub 2013 Jul 11.
1-Desulfoyessotoxin (1-dsYTX) is a desulfated polyether compound belonging to the yessotoxin group of marine toxins. This analogue has been detected in mussels. There are so far no reports on the mechanisms of action of 1-dsYTX in in vitro cell systems. This work evaluates cytotoxic responses in BC3H1 cells exposed to 100 nM 1-dsYTX. The toxicity of 1-dsYTX seems to be similar to that of yessotoxin (YTX). 1-Desulfoyessotoxin induced morphological and biochemical traits typical of a non-apoptotic form of cell death resembling paraptosis. Treated BC3H1 cells showed extensive cytoplasmic vacuolation, enlargement of mitochondria and endoplasmic reticulum and lack of DNA fragmentation. Western blotting analysis revealed phosphorylation of the protein kinase p38 and involvement of the heat shock protein Hsp70. This activation suggests involvement of different signalling pathways for programmed cell death.
1-去硫蛤毒素（1-dsYTX）是一种属于海洋毒素 yessotoxin 族的去磺化聚醚化合物。这种类似物已在贻贝中被检测到。目前尚无关于 1-dsYTX 在体外细胞系统中的作用机制的报告。本工作评估了暴露于 100 nM 1-dsYTX 的 BC3H1 细胞的细胞毒性反应。1-dsYTX 的毒性似乎与 yessotoxin (YTX) 相似。1-去硫蛤毒素诱导了类似于 Paraptosis 的非凋亡细胞死亡的形态和生化特征。经处理的 BC3H1 细胞表现出广泛的细胞质空泡化、线粒体和内质网增大以及无 DNA 片段化。Western blot 分析显示蛋白激酶 p38 的磷酸化和热休克蛋白 Hsp70 的参与。这种激活表明涉及不同的细胞程序性死亡信号通路。
