Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, 50009 Zaragoza, Spain.
FEBS Lett. 2013 Aug 19;587(16):2682-90. doi: 10.1016/j.febslet.2013.07.007. Epub 2013 Jul 9.
In Anabaena sp. PCC 7120, FurA is a global transcriptional regulator whose expression is strongly induced by NtcA in proheterocysts and remains stably expressed in mature heterocysts. In the present study, overexpression of furA partially suppressed heterocyst differentiation by impairing morphogenesis at an early stage. Recombinant purified FurA specifically bound in vitro to the promoter regions of ntcA, while quantitative RT-PCR analyses indicated that furA overexpression strongly affected the transient increase of ntcA expression that occurs shortly after nitrogen step-down. Overall, the results suggest a connection between iron homeostasis and heterocyst differentiation via FurA, by modulating the expression of ntcA.
在鱼腥藻 PCC 7120 中，FurA 是一种全局性转录调控因子，其表达受 NtcA 强烈诱导，在成熟异形胞中稳定表达。在本研究中，furA 的过表达通过在早期阶段损害形态发生而部分抑制异形胞分化。重组纯化的 FurA 特异性地与 ntcA 的启动子区域结合，而定量 RT-PCR 分析表明，furA 的过表达强烈影响氮骤降后短暂增加的 ntcA 表达。总的来说，这些结果表明，通过调节 ntcA 的表达，铁稳态和异形胞分化之间存在联系。
