Perrot-Minnot Marie-Jeanne, Dion Emilie, Cézilly Frank
Université de Bourgogne, UMR CNRS 6282 Biogéosciences, Equipe Ecologie Evolutive, 6 Boulevard Gabriel, 21000 Dijon, France.
Université de Bourgogne, UMR CNRS 6282 Biogéosciences, Equipe Ecologie Evolutive, 6 Boulevard Gabriel, 21000 Dijon, France.
Neuropharmacology. 2013 Dec;75:31-7. doi: 10.1016/j.neuropharm.2013.06.028. Epub 2013 Jul 10.
Serotonin modulates reaction to light in many animals. In the freshwater amphipod Gammarus pulex, exogenous administration of serotonin induces a transient reversal of photic behaviour from strong photophobia to photophily. We have elucidated further the neuromodulation of photic behaviour in G. pulex by using in vivo pharmacology and behavioural testing. Using several mammalian 5-HT receptor antagonists and agonists, we provide evidence for a role of serotonin receptors in the 5-HT-dependent regulation of G. pulex photic behaviour, possibly involving 5-HTR2 subtype. Serotonin-induced photophily was blocked by the 5-HT receptor antagonists, mianserin, cyproheptadine, and ritanserin, but not by ketanserin and methiothepin. In addition, two agonists at 5-HT receptor, mCPP and α-methylserotonin, partially mimicked 5-HT-induced photophily. The natural photophobia of G. pulex was also decreased by exogenous administration of the histamine receptor antagonist cimetidine, suggesting that photic behaviour may be controlled by more complex interactions between serotonergic and histaminergic systems.
血清素调节许多动物对光的反应。在淡水双栖动物蚤状溞中，外源性给予血清素会导致光行为从强烈的畏光暂时转变为嗜光。我们通过体内药理学和行为测试进一步阐明了蚤状溞光行为的神经调节。使用几种哺乳动物5-羟色胺受体拮抗剂和激动剂，我们提供了证据表明血清素受体在5-羟色胺依赖的蚤状溞光行为调节中起作用，可能涉及5-羟色胺受体2亚型。血清素诱导的嗜光被5-羟色胺受体拮抗剂米安色林、赛庚啶和利坦色林阻断，但未被酮色林和甲硫噻平阻断。此外，5-羟色胺受体的两种激动剂，mCPP和α-甲基血清素，部分模拟了血清素诱导的嗜光。组胺受体拮抗剂西咪替丁的外源性给药也降低了蚤状溞的天然畏光性，这表明光行为可能受血清素能和组胺能系统之间更复杂的相互作用控制。
