Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
Anal Biochem. 2013 Oct 1;441(1):32-7. doi: 10.1016/j.ab.2013.06.017. Epub 2013 Jul 11.
Real-time analyses of hepatocellular carcinoma were performed in living mice to assess the applicability of probe electrospray ionization-mass spectrometry (PESI-MS) in medical diagnosis. The number of peaks and the abundance of ions corresponding to triacylglycerols (TAGs) were higher in cancerous tissues than in noncancerous tissues. Multiple sequential scans of the specimens were performed along a predetermined line extending over the noncancerous region to detect the boundary of the cancerous region. Our system successfully discriminated the noncancerous and cancerous tissues based on the intensities of the TAG ions. These results highlight the potential application of PESI-MS for clinical diagnosis in cancer.
在活体小鼠中进行了肝细胞癌的实时分析，以评估探针电喷雾电离-质谱（PESI-MS）在医学诊断中的适用性。与正常组织相比，癌组织中三酰基甘油（TAG）的峰数和离子丰度更高。沿着预先确定的延伸到正常组织区域的线对标本进行多次连续扫描，以检测癌组织的边界。我们的系统成功地基于TAG 离子的强度区分了正常组织和癌组织。这些结果突出了 PESI-MS 在癌症临床诊断中的潜在应用。
