Fu Siqi, Fan Jincai, Liu Liqiang, Jiao Hu, Gan Cheng, Tian Jia, Chen Wenlin, Yang Zengjie, Yin Zhuming
Ninth Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing, China.
J Craniofac Surg. 2013 Jul;24(4):1431-5. doi: 10.1097/SCS.0b013e31828dcc1f.
Both in vivo and in vitro, cells are subjected to mechanical stress, which can affect their proliferation and differentiation. However, the detailed mechanisms of the accompanying morphologic and biochemical changes remain elusive. This is partially caused by the lack of suitable stretch model and method that mimic soft tissue expansion used in plastic surgery. In this study, we report the development of a novel model for uniaxial stretch cell mimicking tissue expansion.
Fibroblasts, grown in plastic culture caskets, were stretched in a progressive and accumulative manner lasting from 1 to 4 days. Cell proliferation was measured using the methyl thiazolyl tetrazolium assay and the Western blot analysis of cell cycle proteins.
Following the stretch protocol, fibroblasts elongated and aligned parallel to each other and to the applied strain vectors. Furthermore, stretch-stimulated cell proliferation was compared with the unstretched conditions.
These results demonstrate that this uniaxial cell stretcher device and the accumulative stretch method are a suitable system for mimicking the tissue expansion process used in clinical applications.
Not gradable.
在体内和体外，细胞都会受到机械应力的作用，这会影响它们的增殖和分化。然而，伴随的形态学和生化变化的详细机制仍然不清楚。部分原因是缺乏模拟整形外科中使用的软组织扩张的合适拉伸模型和方法。在本研究中，我们报告了一种用于模拟组织扩张的新型单轴拉伸细胞模型的开发。
在塑料培养盒中生长的成纤维细胞以渐进和累积的方式拉伸，持续1至4天。使用甲基噻唑基四氮唑法和细胞周期蛋白的蛋白质印迹分析来测量细胞增殖。
按照拉伸方案，成纤维细胞彼此平行并与施加的应变向量对齐并伸长。此外，将拉伸刺激的细胞增殖与未拉伸条件进行比较。
这些结果表明，这种单轴细胞拉伸装置和累积拉伸方法是模拟临床应用中使用的组织扩张过程的合适系统。
不可分级。
