[雌二醇对大鼠下颌髁突软骨细胞雌激素受体表达的影响]
[Effect of estradiol on the expression of estrogen receptor in mandibular condylar cartilage cell of rat].
作者信息
Hong Lu, Chen Chuan-jun
机构信息
Department of Oral and Maxillofacial Surgery, the Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2013 Jun;22(3):278-81.
PURPOSE
To evaluate the effect of estradiol on the expressions of estrogen receptor in mandibular condylar cartilage cell (MCC).
METHODS
The MCC were harvested from neonatal rats. The experimental groups was cultured in medium with different concentration(10(-3),10(-6),10(-9),l0(-12) mmol/L) of estradiol(E2), while the control group was cultured in medium with ethanol. The ERα mRNA and ERβ mRNA in cells were detected with RT-PCR.The data was processed with SPSS10.0 software package.
RESLUTS
Both ERα and ERβ expression were observed in MCC. Exogenous 17β-E2 raised the gene expression of ERβ and lowered the expression of ERα. Under the concentration of 10-9 mmol/L(physiological condition), the up-regulation of ERα mRNA was found most obviously.
CONCLUSIONS
E2 can raise the expression of ERα and decrease the expression of ERβ, and the effect of up grading is the most obvious under physiological concentration.
目的
评估雌二醇对下颌髁突软骨细胞（MCC）中雌激素受体表达的影响。
方法
从新生大鼠中获取MCC。实验组在含有不同浓度（10⁻³、10⁻⁶、10⁻⁹、10⁻¹² mmol/L）雌二醇（E2）的培养基中培养，而对照组在含有乙醇的培养基中培养。用逆转录聚合酶链反应（RT-PCR）检测细胞中的ERα mRNA和ERβ mRNA。数据用SPSS10.0软件包处理。
结果
在MCC中观察到ERα和ERβ的表达。外源性17β-E2提高了ERβ的基因表达并降低了ERα的表达。在10⁻⁹ mmol/L（生理条件）浓度下，ERα mRNA的上调最为明显。
结论
E2可提高ERα的表达并降低ERβ的表达，且在生理浓度下上调作用最为明显。
Zotero 插件