Yildirim-Bicer Arzu Zeynep, Ergun Gulfem, Egilmez Ferhan, Demirkoprulu Hisam
Department of Prosthodontics, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Indian J Dent Res. 2013 Jan-Feb;24(1):81-6. doi: 10.4103/0970-9290.114962.
The aim of this study was to compare the cytotoxic effects of two indirect composite resins (Artglass and Solidex) on the viability of L-929 fibroblast cells at different incubation periods by storing them in artificial saliva (AS).
Disk-shaped test samples were prepared according to manufacturers' instructions. Test materials were cured with light source (Dentacolor XS, Heraus Kulzer, Germany). The samples were divided into two groups. The first group's samples were transferred into a culture medium for 1 hour, 24 hours, 72 hours, 1 week and 2 weeks. The other group's samples were transferred into a culture medium for 1 hours, 24 hours, 72 hours, 1 week, and 2 weeks after being stored in AS for 48 hours. The eluates were obtained and pipetted for evaluation onto L-929 mouse fibroblast cultures incubated for 24 hours. Measurements were performed by MTT (3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The degree of cytotoxicity for each sample was determined according to the reference values represented by the cells with a control group.
Statistical significance was determined by ANOVA. Both groups presented lower cell viability in comparison to the control group at all periods. Storing in artificial saliva reduced cytotoxicity significantly (P < 0.05). Stored Artglass and Solidex showed similar effects on cytotoxicity. Nonstored Solidex samples were found more cytotoxic than Artglass samples. The cell survival rate results of 24-hour incubation period were significantly lower than those of the other experimental periods (P < 0.05).
Storing indirect composite resins in AS may reduce cytotoxic effects on the fibroblast cells. However, resin-based dental materials continue to release sufficient components to cause cytotoxic effects in vitro after 48 hours of storing in AS.
本研究旨在通过将两种间接复合树脂（Artglass和Solidex）储存在人工唾液（AS）中，比较它们在不同孵育期对L-929成纤维细胞活力的细胞毒性作用。
根据制造商的说明制备圆盘形测试样品。测试材料用光源（Dentacolor XS，德国贺利氏古莎公司）固化。样品分为两组。第一组样品转移至培养基中培养1小时、24小时、72小时、1周和2周。另一组样品在AS中储存48小时后转移至培养基中培养1小时、24小时、72小时、1周和2周。获取洗脱液并移液用于对孵育24小时的L-929小鼠成纤维细胞培养物进行评估。通过MTT（3-(4,5)-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法进行测量。根据对照组细胞代表的参考值确定每个样品的细胞毒性程度。
采用方差分析确定统计学意义。在所有时期，两组与对照组相比细胞活力均较低。储存在人工唾液中可显著降低细胞毒性（P<0.05）。储存后的Artglass和Solidex对细胞毒性表现出相似的作用。未储存的Solidex样品比Artglass样品的细胞毒性更大。24小时孵育期的细胞存活率结果显著低于其他实验时期（P<0.05）。
将间接复合树脂储存在AS中可能会降低对成纤维细胞的细胞毒性作用。然而，树脂基牙科材料在AS中储存48小时后仍会释放足够的成分，在体外引起细胞毒性作用。
