Bal Bilge Turhan, Sönmez Nalan, Bavbek Barkin, Akçaboy Cihan
Gazi University, Faculty of Dentistry, Department of Prosthodontics, Emek, Ankara, Turkey.
N Y State Dent J. 2011 Apr;77(3):31-5.
The aim of this study was to compare the cytotoxic effects of expired and non-expired resin-based composite materials at two, five and seven days on L929 cells. Disc-shaped test samples of expired and non-expired composite materials were prepared according to manufacturers' instructions. The samples were transferred into a culture medium for 24 hours. Eluates were obtained and pipetted onto L-929 mouse fibroblast cultures and incubated for evaluation after two, five and seven days. After each of the incubation periods, measurements were performed by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control. Statistical significance was determined by two-way analysis of variance, followed by Duncan's test. Among all composite materials, expired composites presented lower cell viability than the non-expired composites over the three-day time period; the difference in survival rates between the expired and non-expired groups was statistically significant (P < 0.05). Non-expired Quadrant Universal LC demonstrated high survival rates, followed by Filtek Z250 and Alpha-dent at two and five days. It can be concluded that expiration dates have a significant effect on the cytotoxicity of the composite materials.
本研究的目的是比较过期和未过期的树脂基复合材料在两天、五天和七天时对L929细胞的细胞毒性作用。根据制造商的说明制备过期和未过期复合材料的圆盘形测试样品。将样品转移到培养基中24小时。获取洗脱液并吸取到L-929小鼠成纤维细胞培养物上，在两天、五天和七天后进行孵育以进行评估。在每个孵育期后，通过MTT法进行测量。根据对照细胞代表的参考值确定每个样品的细胞毒性程度。通过双向方差分析，随后进行邓肯检验确定统计学意义。在所有复合材料中，在三天的时间段内，过期复合材料的细胞活力低于未过期复合材料；过期组和未过期组之间的存活率差异具有统计学意义（P < 0.05）。未过期的Quadrant Universal LC显示出高存活率，其次是Filtek Z250和Alpha-dent在两天和五天时。可以得出结论，保质期对复合材料的细胞毒性有显著影响。
