Department of Sports Medicine for Health Promotion, Tokyo Medical University, Tokyo, Japan.
Faculty of Sport and Health Science, Ritsumeikan University, Kyoto, Japan.
Adv Exp Med Biol. 2013;789:163-169. doi: 10.1007/978-1-4614-7411-1_23.
Recently, deoxygenated hemoglobin (HHb) has been used as one of the most popular indicators of muscle O2 extraction during exercise in the field of exercise physiology. However, HHb may not sufficiently represent muscle O2 extraction, as total hemoglobin (tHb) is not stable during exercise. The purpose of this study was to measure various muscle oxygenation signals during cycle exercise and clarify which is the best indicator of muscle O2 extraction during exercise using NIRS. Ten healthy men performed 6-min cycle exercise at both moderate and heavy work rates. Oxygenated hemoglobin (O2-Hb), HHb, tHb, and muscle tissue oxygen saturation (SmO2) were measured with near-infrared spatial resolved spectroscopy from the vastus lateralis muscle. Skin blood flow (sBF) was also monitored at a site close to the NIRS probe. During moderate exercise, tHb, O2-Hb, and SmO2 displayed progressive increases until the end of exercise. In contrast, HHb remained stable during moderate work rate. sBF remained stable during moderate exercise but showed a progressive decrease at heavy work rate. These results provide evidence that HHb may not sufficiently represent muscle O2 extraction since tHb is not stable during exercise and HHb is insensitive to exercise-induced hyperaemia.
近年来，去氧血红蛋白（HHb）已被用作运动生理学领域运动期间肌肉氧提取的最受欢迎指标之一。然而，HHb可能不能充分代表肌肉氧提取，因为总血红蛋白（tHb）在运动过程中不稳定。本研究的目的是使用近红外光谱空间分辨技术测量循环运动期间的各种肌肉氧合信号，并阐明在运动期间哪种是肌肉氧提取的最佳指标。10 名健康男性以中等和高强度进行 6 分钟的自行车运动。从股外侧肌用近红外空间分辨光谱测量含氧血红蛋白（O2-Hb）、HHb、tHb 和肌肉组织氧饱和度（SmO2）。在靠近 NIRS 探头的部位还监测皮肤血流量（sBF）。在中等强度运动期间，tHb、O2-Hb 和 SmO2 显示出逐渐增加，直到运动结束。相比之下，HHb 在中等工作速率下保持稳定。sBF 在中等运动期间保持稳定，但在高强度运动时逐渐下降。这些结果表明 HHb 可能不能充分代表肌肉氧提取，因为 tHb 在运动过程中不稳定，HHb 对运动诱导的充血不敏感。
